2022 / 02

Next Paper
DOI: 10.24075/brsmu.2022.012

ORIGINAL RESEARCH

Activity of nuclear factor κB in lymphocyte populations of children with psoriasis

Kuptsova DG1, Petrichuk SV1, Murashkin NN1,2,3, Kurbatova OV1, Radygina TV1, Khotko AA2, Ivanov RA1
About authors

1 National Medical Research Center for Children's Health, Moscow, Russia

2 Central State Medical Academy of the Department of Presidential Affairs of the Russian Federation, Moscow, Russia

3 Sechenov First Moscow State Medical University, Moscow, Russia

Correspondence should be addressed: Darya G. Kuptsova
Lomonosovsky prospect, 2, str. 1, Moscow, 119296; moc.liamg@avostpuk.gd

About paper

Finding: the study was part of the state assignment for the Ministry of Health of the Russian Federation, № АААА-А19-119013090093-2.

Acknowledgements: the authors wish to thank all the patients for active cooperation and express their thanks to the researchers of the Laboratory of Immunology and Virology, as well as to dermatologists and nurses at the Dermatology Department, National Medical Research Center for Children's Health, Moscow, Russia, who contributed to the study.

Author contribution: Kuptsova DG, Petrichuk SV — study concept and design, experimental data acquisition and analysis, statistical analysis, manuscript writing and editing; Kurbatova OV, Radygina TV — experimental data acquisition, manuscript editing; Murashkin NN, Khotko AA, Ivanov RA — data analysis, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the National Medical Research Center for Children's Health (protocol № 2 dated February 14, 2020), conducted in accordance with the principles of the Declaration of Helsinki, and registered with ClinicalTrials.gov ID: NCT04989296. Parents of all children and adolescents enrolled submitted the informed consent to medical intervention in hospital settings, personal data processing and the use of data for scientific purposes.

Received: 2022-02-11 Accepted: 2022-03-03 Published online: 2022-03-20
|
Fig. 1. Stages of analysis when quantifying cells with NF-κB translocation. A. Gallery of recorded cell images. B. Isolation of cells in good focus based on Gradient RMS. C. Isolation of single events. D. Isolation of the studied lymphocyte population, particularly CD3+CD4+ (CD3_ECD — channel 4; CD4_PB — channel 3). E. Isolation of doublepositive cells: NF-κB+/7-AAD+ (NF-κB_FITC — channel 2; 7-AAD — channel 5). F. Isolation of the proportion of cells with NF-κB translocation based on Similarity>1
Fig. 2. Number of major (А) and minor (B) populations of lymphocytes and NK cells with NF-κB translocation in children with psoriasis and comparison group.
р — significance of differences between populations, Wilcoxon signed rank test for dependent variables
Fig. 3. Multiple regression equation for the relationship between PASI (А) and BSA (B) scores and the number of cells with NF-κB translocation in children with psoriasis, and the relationship between the observed and predicted values calculated using the regression eqution for PASI (C) and BSA (D) indices. Regression equations were constructed using the stepwise multiple regression method with exceptions. Rmul — coefficient of multiple correlation, R; р — significance of non-zero regression coefficients
Table 1. Number of cells with NF-κB translocation in children with psoriasis and comparison group
Note: р — significance of differences between groups, Mann–Whitney U test.
Table 2. Correlations of the number of cells with NF-κB translocation in lymphocyte populations with the patients' age and the duration of psoriasis
Note: r — Pearson correlation coefficient, p — probability of non-zero regression coefficients.
Table 3. Number of cells with NF-κB translocation in children with psoriasis before and 24 h after administration of GEBD
Note: р — significance of differences in the numbers of cells with NF-κB translocation obtained before and 24 h after administration of GEBD, Mann–Whitney U test.