We investigated whether single nucleotide polymorphism (SNP) 49A>G (rs231775) in CTLA4 gene was associated with multiple sclerosis (MS) susceptibility and MS clinical features, such as disease severity estimated by MSSS scale, onset symptoms and the duration of the first remission. DNA samples of 508 multiple sclerosis (MS) patients and 210 healthy individuals (all individuals were Russians by ethnicity) were analyzed. Genotyping was performed using PCR method with the analysis of restriction fragment length polymorphism. No association of SNP rs231775 with MS susceptibility was found. After stratification of patients according to their first remission duration reliable associations of G allele carriage with the short first remission (<1 year; p = 0.044, odds ratio = 1.5), and A/A genotype carriage with the long first remission (>1 year) were observed. No reliable associations of SNP rs231775 with MS severity or onset symptoms were revealed. Carriage of CTLA4*G allele may be used as the prognostic marker for evaluation of subsequent relapses frequency in MS patients.