ISSN Print 2500–1094    ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
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Original research Development of the vascular condition classifier using supervised machine learning methods
Besedovskaia ZV, Korobov A, Kudriashova N
Quantitative processing of optoacoustic angiograms is an important task, the solution of which will potentially enable the early diagnosis of vascular diseases. The aim of this study is to refine and conduct biomedical validation of a software tool for the analysis of optoacoustic angiograms, focusing on the application of machine learning methods. The work was conducted on an open dataset containing three-dimensional optoacoustic angiograms of an experimental animal (mouse) in three temperature conditions: cold temperature (16 °C), room temperature (23 °C), and body temperature (30 °C), as well as a dataset with basic vascular features obtained by processing using Amira/Avizo (Thermo Fisher Scientific), a general-purpose software for visualization and analysis of scientific and industrial data. Various vascular features missing from previous work were developed and calculated, after which basic methods of unsupervised/supervised clustering and supervised classification were applied to determine different temperature conditions of vessel segments. Supervised classification methods demonstrated high overall accuracy: CatBoost — 98.9%, SGDClassifier — 95.7%, and logistic regression — 99.7%. The results are consistent with existing descriptions of vascular changes during temperature tests. The applied methodology is universal, meaning with minor modifications it can be adapted to patients. Therefore, the results of this study may potentially improve the diagnosis of vascular pathologies.
Received: 2025-10-07
Published online: 2025-11-27
DOI: 10.24075/brsmu.2025.060
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VIEWS 135
Original research Comparative imaging of the HAEE-CY5 and ЕЕАН-СУ5 tetrapeptide binding to the Aβ aggregates on the SH-SY5Y cells
Ivanova AV, Chmelyuk NS, Kuzmichev IA, Shilyaeva MI, Abakumov MA
Due to high diagnostic value of β-amyloid aggregates, the target ligands capable of specific binding to abnormal Aβ aggregates are of special interest. The study aimed to perform comparative characterization of the HAEE-Сy5 (Ac-His-Ala-Glu-Glu-Gly-Gly-Gly-Gly-Lys(ε-Cy5)-NH2) and EEAH-Cy5 (Ac-Glu-Glu-Ala-His-GlyGly-Gly-Gly-Lys(ε-Cy5)-NH2) tetrapeptide capability of binding to the Aβ aggregates in the SH-SY5Y human neuroblastoma cell line by confocal microscopy. It has been shown that the HAEE-Cy5 tetrapeptide demonstrates specific binding yielding typical cytoplasmic clusters and clear co-localization with the amyloid aggregates, while the EEAH-Cy5 peptide with the inverted sequence totally loses the binding capability. Quantification has confirmed high specificity of the HAEECy5 binding to the Aβ aggregates (Manders' colocalization coefficient 0.58 ± 0.03). It has been found that the histidine N-terminal position is a critical determinant of the interaction specificity. The findings offer the prospects of using the HAEE peptide as a platform for the development of targeted disgnostic systems for amyloid disorder imaging.
Received: 2025-10-15
Published online: 2025-11-24
DOI: 10.24075/brsmu.2025.061
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VIEWS 149
Original research Perivascular mast cells and angiogenesis in the tumor microenvironment of synovial sarcoma
Bulanov DV, Makhachev DR, Suntsov MA, Gubich DS, Filippova YD, Krutilina AA, Svoyak AV, Ivannikova AA, Ghabibullaev RM
Synovial sarcoma is characterized by marked histological and molecular heterogeneity, and angiogenesis as well as innate immune cells are considered potential sources of prognostic markers and therapeutic targets. This study aimed to evaluate the relationship between the quantitative and spatial characteristics of mast cells and angiogenesis in the tumor microenvironment of synovial sarcoma, as well as their prognostic significance. Using immunohistochemistry (tryptase/ CD117, CD31/CD34, VEGF-A, α-SMA, CD3/CD8, CD68/CD163) and digital morphometry normalized to 1 mm2, we analyzed 140 cases of synovial sarcoma. The intrathumoral, peritumoral, and perivascular (≤50 µm from CD31+/CD34+ vessels) zones, as well as the mastocyte degranulation index, were evaluated separately. Mast cells were detected in all observations; their density and signs of degranulation were greatest in the perivascular zone. Perivascular mast cells were positively correlated with both microvascular density and VEGF-A expression, and inversely correlated with α-SMA pericyte coverage; these relationships remained significant even after accounting for CD163+ macrophages. A high microvascular density and increased perivascular mast cell counts were associated with an unfavorable survival prognosis, while pronounced CD8+ infiltration predicted better outcomes. The developed integral Mast-Angio Score, which combines perivascular density, mast cell degranulation, microvascular density, and VEGF-A expression, improves the accuracy of prognostic stratification and can serve as a morphological basis for justifying combined antiangiogenic and immune therapy.
Received: 2025-10-07
Published online: 2025-11-21
DOI: 10.24075/brsmu.2025.059
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VIEWS 180
Clinical case Severe acute α-PVP poisoning complicated by systemic rhabdomyolysis syndrome: case report
Makarovskaya NP, Lodyagin AN, Batocyrenov ChB, Razamasova SYu, Antonova AM, Narzikulov RA, Sokolova ES
Today, acute poisoning with modern psychoactive substances and narcotic drugs is among the major causes of emergency admissions to toxicology units. There is a clear upward trend in the number of life-threatening complications of poisoning with psychoactive substances, one of which is rhabdomyolysis. Here we report a clinical case of severe acute α-PVP poisoning complicated by systemic rhabdomyolysis, describe the features of the clinical course and intensive care for this disorder. The early diagnosis, timely and effective treatment by conducting aggressive detoxification infusion therapy made it possible to prevent progression to the anuric phase of acute kidney injury, as well as to avoid invasive procedures of extracorporeal detoxification methods and probably avoid the adverse outcome of the acute α-PVP poisoning complicated by systemic rhabdomyolysis.
Received: 2025-10-02
Published online: 2025-11-19
DOI: 10.24075/brsmu.2025.058
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VIEWS 160
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Original research Analysis of the Russians' awareness of bone marrow donation and the Federal Bone Marrow Donor Registry infrastructure
Butunts MA, Dyuzhina KA, Nifatova ES, Muradyan TG
Bone marrow transplant is the leading method to treat hematologic malignancies, immunodeficiency, and hereditary metabolic disorders. The Federal Bone Marrow Donor Registry effectiveness depends directly on public awareness of bone marrow donation and infrastructure development. A comprehensive approach to public awareness is necessary to increase the system effectiveness. The study aimed to investigate factors that influence joining the Federal Bone Marrow Donor Registry, with a focus on motivation, sources of information, impact of infrastructure, environment, and common myths. The respondents (potential donors registered in the Federal Registry; n = 3100) filled an online questionnaire of 24 questions aimed at studying and assessing the socio-demographic characteristics, motivation, sources of information, influence of the environment, awareness of bone marrow donation, and readiness to donate. It was found that young adults aged 18–36 (n = 1860) more often join the Federal Registry through informal channels, such as work/school events (n = 843; 27.2%), while respondents over the age of 37 (n = 1240) prefer healthcare institutions (n = 1590; 51.3%). Women make up the majority of potential donors (n = 2304; 74.3%), especially in Moscow (n = 1650; 74.5%), while higher prevalence of myths is reported for the regions (n = 1646; 53.1%). The findings emphasize the need for the differentiated approach to information policy, which will make it possible to increase the donor movement effectiveness nationwide. A key factor in scaling this work is partnership with commercial laboratories, which significantly expands the Federal Registry recruitment network and provides convenient conditions for donors to join.
Received: 2025-05-26
Published online: 2025-06-26
DOI: 10.24075/brsmu.2025.032
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VIEWS 923
Original research Comparative pharmacokinetics and biodistribution of HAEE and HASS peptides
Ivanova AV, Lazareva PA, Kuzmichev IA, Vadekhina VV, Kosykh AV, Gurskaya NG, Abakumov MA
Due to the limited availability of advanced methods to diagnose Alzheimer's disease, small molecules and short peptides capable of specifically biniding β-amyloid are of special interest. The study aimed to perform comparative assessment of pharmacokinetics and biodistribution of two model peptides, HAEE (His-Ala-Glu-Glu) and HASS (His-Ala-Ser-Ser), as potential platforms for the development of diagnostic kits, as well as to determine the relationship between the peptide structure and organotropism. The study involving BALB/c mice (n = 10) was conducted using the fluorescence labeled compounds. We used in vivo IVIS imaging, ex vivo fluorescence microscopy, and pharmacokinetic analysis. The results showed fundamental differences: the negatively charged HAEE was accumulated mainly in the kidney (T½β = 4.39 h) due to tubular reabsorption, while neutral HASS was soon captured by the liver (T½β = 2.76 h). The data obtained demonstrate the key role of amino acid composition in determining organotropism and open prospects for the development of organ-specific peptide systems.
Received: 2025-07-10
Published online: 2025-07-31
DOI: 10.24075/brsmu.2025.036
Comments 0
VIEWS 888
Special Project Author's Work
Dear researcher!
At the end of 2015, Bulletin of RSMU saw an important change in its typographic design and content. We formulated new editorial policies and established strict ethical standards for submitted manuscripts in accordance with the guidelines of reputable international bodies. As a result, about a quarter of the submitted works have been rejected, the primary reason being the author trying to submit a previously published article. Sometimes authors believe that by making slight changes to the introduction, excluding a few people from the study, performing a new statistical analysis, and thus obtaining totally new results they will turn their old manuscript into a novel work. That is why we would like to talk about scientific integrity, honesty, plagiarism, and self-plagiarism in our special project “Author’s work”.
Richard FEYNMAN Cargo cult science
American physicist Richard P. Feynman, a Nobel laureate, was always very scrupulous about the quality of a research study. During his commencement address at the California Institute of Technology in 1974, he talked about scientific integrity and honesty and warned young researchers “not to fool” themselves. A must-read for anyone who believes he/she is a true scientist.
Ivan PAVLOV On the Russian mind
In 1918, Russian physiologist Ivan Pavlov, a Nobel laureate, delivered two lectures: on the mind in general and the Russian mind in particular; on those mind qualities that determine the success of a research work and on how these qualities are present in the Russian mind. Pavlov's thoughts are an effective vaccine against poor intellectual work.
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