Bulletin of RSMU

ISSN Print 2500–1094 ISSN Online 2542–1204

Bulletin of RSMU

BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)

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Issue 1, 2022

Topic Red king crab nuclease applications

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Issue 2, 2021

Topic Compulsory licensing in pharmaceutical industry

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Issue 3, 2021

Topic Persistence of SARS-CoV-2 in human corpses

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Issue 4, 2021

Topic Dermatology and telemedicine: goals, advantages and disadvantages

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Issue 5, 2021

Topic AAV mediated delivery in gene therapy

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Issue 6, 2021

Topic Parkinson’s disease

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New articles

Vorobyeva IV, Bulava EV, Moshetova LK, Pinchuk AV

Simultaneous pancreas and kidney transplantation (SPK) provides effective treatment in patients with type 1 diabetes mellitus (T1DM) and end-stage renal failure (ESRF), mitigating the hyperglycemia and uremic syndrome. The study aimed at the assessment of morphofunctional status of the macula and macular hemodynamics in patients with T1DM after SPK. The study enrolled 45 patients subdivided into three groups: Group A — patients with T1DM after SPK; Group B — patients with T1DM and ESRF, maintained on programmed hemodialysis (PH), on waiting list for SPK; and Group C — individuals without ophthalmic or systemic pathologies. All patients were subject to the standard ophthalmological examination complemented by measurements of the central retinal thickness (CRT) and the average perfusion density (PD) in four vascular layers: superficial capillary plexus of the retina (SCP), deep capillary plexus of the retina (DCP), choriocapillaris, and choroid. The patients after SPK had significantly lower CRT (241 ± 33 µm in Group A, 309±10 µm in Group B; p < 0.05) and significantly higher PD of the macular region in both the retina (Group A: SCP — 19.0 ± 1.6%, DCP — 10.7 ± 1.3%; Group B: SCP — 11.7 ± 0.8%, DCP — 4.8 ± 0.8%; p < 0.05) and the choroid (Group A: choriocapillaris — 28.1 ± 1.8%, choroid — 31.3 ± 1.6%; Group B: choriocapillaris — 20.4 ± 1.6%, choroid — 21.8 ± 1.3%; p < 0.05), as well as significantly higher visual acuity (Group A: 0.7 ± 0.1; Group B: 0.5 ± 0.1; p < 0.05) and macular light threshold (Group A: 25.9 ± 1.4 dB; Group B: 22.3 ± 1.1 dB; p < 0.05) compared with the patients on PH. Thus, the normalization of carbohydrate metabolism and the mitigation of uremic syndrome in patients with T1DM and ESRF after SPK favorably affect the functional condition of the macular area, as indicated by the improvement in macular blood flow and visual functions.

Received: 2022-02-22

Published online: 2022-04-05

DOI: 10.24075/brsmu.2022.013

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VIEWS 34

Kuptsova DG, Petrichuk SV, Murashkin NN, Kurbatova OV, Radygina TV, Khotko AA, Ivanov RA

Alterations in intracellular signaling pathways affecting immune cell activation, proliferation and differentiation of keratinocytes in psoriasis could explain the complex pathogenesis of the disease. NF-κB is one of the intracellular signaling pathways, which is involved in regulation of numerous pro-inflammatory genes, and affects the synthesis of pro-inflammatory cytokines directly involved in the development of psoriasis. The study was aimed to assess the number of cells with NF-κB translocation in lymphocyte populations of children with psoriasis depending in the disease severity and therapy. A total of 130 children with psoriasis vulgaris were examined. The comparison group included 30 healthy children. The study was conducted using the imaging flow cytometry Amnis ImageStreamX system. It was found that there were significant differences in the number of cells with NF-κB translocation in the lymphocyte populations of both children with psoriasis and comparison group. Children with psoriasis had a higher number of cells with NF-κB translocation in the populations of T helper cells, Tact, Treg, and Th17 compared to healthy children (p < 0.05). The number of cells with NF-κB translocation in children with psoriasis correlated with the disease severity PASI (Rmul = 0.32) and BSA (Rmul = 0.31) scores, as well as with the disease duration (p < 0.05). NF-κB determination could be considered an additional criterion for the disease severity assessment in children with psoriasis. The differences in the degree of reduction of the number of cells with NF-κB translocation 24 h after administration of biologics (adalimumab, etanercept, ustekinumab) have been shown. Studying NF-κB in cell populations offers the prospect of understanding pathogenetic mechanisms of inflammation and developing new therapeutic methods for psoriasis.

Received: 2022-02-11

Published online: 2022-03-20

DOI: 10.24075/brsmu.2022.012

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VIEWS 121

Nikishina VB, Petrash EA, Prirodova OF, Akhramenko RS, Danilova AV, Kuznetsova AA

Optimization of the educational process, including distance learning, requires orderly arrangement of the information presented, which translates into the need to factor in oculomotor reactions accompanying the search for solutions to simple cognitive tasks. This need supports the relevance of the present study, which aimed to investigate the age-dependent parameters of the oculomotor reactions occurring in solving a simple cognitive task. The sample included 97 persons, 47 males and 50 females, ages 21 to 36. For the purpose of rating the oculomotor reactions, the sample was divided into age groups: 21–26 years (n = 34); 27–32 years (n = 29); 33–36 years (n = 34). The methodology that governed the rating procedures was developed by the authors of the study and relied on the Tobii EyeX eye tracking hardware and software solution (GazeControl software). The study revealed a significant correlation between orientation of the image (presentation angle) and distribution of the visual fixations: regardless of the image presentation angle and its properties (schematic monochrome or full color image), the fixations tend to fall predominantly into the top and left parts of the image (the first quadrant). Other findings include a) a significant dependence of the capability to solve simple cognitive tasks and recognize the contents of the image on the spatial orientation of the presented stimuli, and b) the number of errors made in image contents recognition increasing with age, this dependence being significant and observed for both the schematic monochrome image (p1 = 0.014; p2 = 0.016; p3 = 0.014) and the full-color image (p1 = 0.015; p2 = 0.015; p3 = 0.017). The researchers have also identified the significant angles of rotation of the presented face images that stably caused recognition errors.

Received: 2022-02-07

Published online: 2022-03-18

DOI: 10.24075/brsmu.2022.011

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VIEWS 104

Shagin DA, Rebrikov DV

Duplex-specific nuclease (DSN) from hepatopancreas of the craboid Paralithodes camtschaticus (red king crab) has a unique combination of properties. Along with thermal stability and a high optimal temperature of catalysis, this enzyme exhibits high substrate selectivity, cleaving only DNA in duplexes (DNA-DNA or DNA-RNA). Accordingly, it digests neither single strands (nor single-stranded regions) of DNA, nor RNA strands with any secondary structure. Such properties make it possible to create unique protocols based on DSN, which is also an important object of fundamental research in the field of nuclease evolution. The review considers diverse applications of the red king crab DSN in modern methods of molecular biology.

Received: 2022-02-22

Published online: 2022-02-28

DOI: 10.24075/brsmu.2022.010

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VIEWS 150

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Popular articles

Voroshilina ES, Zornikov DL, Ivanov AV, Pochernikov DG, Panacheva EA

The analysis of semen microbiota is difficult due to the lack of established criteria for interpretation of microbiological tests. The aim of the study was to determine the stable clusters of semen microbiota analyzed by real-time PCR in samples with normozoospermia. Semen samples of 227 men with normal spermiograms were included in the study. The quantity of total bacterial DNA and at least one group of microorganisms was more than 103 GE/ml in 107 (41.7%) samples. Four stable microbiota clusters with the prevalence of a specific microorganism group were distinguished in these samples: obligate anaerobes (OA) cluster (proportion in the centroid — 81.1%); Lactobacillus spp. cluster (proportion in the centroid — 64.3%); gram-positive facultative anaerobes (GPFA) cluster (proportion in the centroid — 92.5%); Enterobacteriaceae/Enterococcoccus (EE) cluster (proportion in the centroid — 80.8%). The clusters were ranked by frequency of occurrence: OA cluster was the most prevalent (43 (40.2%) of 107), second-most frequent were GPFA-cluster (27 (25.2%)) and Lactobacillus-cluster (22 (20.6%)). EE-dominated cluster was found in 15 (14.0%) cases.

Received: 2021-09-16

Published online: 2021-10-28

DOI: 10.24075/brsmu.2021.048

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VIEWS 755

Kolobukhina LV, Burgasova OA, Kruzhkova IS, Bakalin VV, Generalova LV, Shagaev AV, Ogarkova DA, Nikiforova MA, Vasina DV, Guschin VA, Smetanina SV

The COVID-19-associated mortality remains high. Studying the features of the COVID-19 course in vaccinated patients, who have got ill on different dates after vaccination, compared to unvaccinated individuals is relevant. The study was aimed to assess clinical and immunological features of the COVID-19 course, as well as to assess humoral immunity (virus neutralizing activity, VNA) and SARS-CoV-2 S protein RBD domain variation in the groups of patients, previously vaccinated with Sputnik V, and unvaccinated patients. A total of 251 patients with confirmed diagnosis of COVID-19 were enrolled, of them 116 individuals were previously vaccinated with one or two Sputnik V vaccine components, and 135 patients were not vaccinated (comparison group). Individuals over 50 years of age prevailed (82.8%). The patients, who received two vaccine components, had mild to moderate COVID-19 (92.1%). In the group of unvaccinated patients, 11 individuals received treatment in the ICU, 10 of them died. The viral load was significantly lower in vaccinated patients. Mutations of SARS-CoV-2, such as S477N, S477N+A522S, E484K and E484K+S494P, were identified both in vaccinated and unvaccinated patients. Assessment of the neutralizing activity of sera revealed no significant differences in VNA against different variants of SARS-CoV-2 mutations. The data obtained demonstrate that the lack of vaccination is an aggravating factor and is capable of increasing the risk of severe course and death in patients with COVID-19.

Received: 2021-09-29

Published online: 2021-10-17

DOI: 10.24075/brsmu.2021.046

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VIEWS 565

Special Project Author's Work

Secrets of a quality research study and a good manuscript

At the end of 2015, Bulletin of RSMU saw an important change in its typographic design and content. We formulated new editorial policies and established strict ethical standards for submitted manuscripts in accordance with the guidelines of reputable international bodies. As a result, about a quarter of the submitted works have been rejected, the primary reason being the author trying to submit a previously published article. Sometimes authors believe that by making slight changes to the introduction, excluding a few people from the study, performing a new statistical analysis, and thus obtaining totally new results they will turn their old manuscript into a novel work. That is why we would like to talk about scientific integrity, honesty, plagiarism, and self-plagiarism in our special project “Author’s work”.

American physicist Richard P. Feynman, a Nobel laureate, was always very scrupulous about the quality of a research study. During his commencement address at the California Institute of Technology in 1974, he talked about scientific integrity and honesty and warned young researchers “not to fool” themselves. A must-read for anyone who believes he/she is a true scientist.

In 1918, Russian physiologist Ivan Pavlov, a Nobel laureate, delivered two lectures: on the mind in general and the Russian mind in particular; on those mind qualities that determine the success of a research work and on how these qualities are present in the Russian mind. Pavlov's thoughts are an effective vaccine against poor intellectual work.