ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
A protocol of development of a screening assay for evaluating immunological memory to vaccine-preventable infections: simultaneous detection of antibodies to measles, mumps, rubella and hepatitis B
About authors
1 Laboratory of Translational Biomedicine,N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow, Russia
2 Institute of Molecular Medicine,I. M. Sechenov First Moscow State Medical University, Moscow, Russia
3 Department of Virology, Faculty of Biology,Lomonosov Moscow State University, Moscow
Correspondence should be addressed: Elena Mazunina
ul. Gamalei, d. 18, Moscow, Russia, 123098; ur.relbmar@aneleanidlan
About paper
Funding: this work was supported by the Russian Ministry of Health.
Contribution of the authors to this work: Mazunina EP, Kleymenov DA — analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, writing of a final version of a manuscript; Manuilov VA — analysis of literature, research planning, drafting of a manuscript; Gushchin VA — analysis of literature, research planning, data analysis and interpretation, drafting of a manuscript, writing of a final version of a manuscript; Tkachuk AP — analysis of literature, research planning, critical analysis of a manuscript.
Received: 2017-09-26
Accepted: 2017-10-10
Published online: 2018-01-13
|
Multiplex screening assays for measuring antibodies to vaccine-preventable infections are routinely used in large-scale seroepidemiological studies, but not commercially available, because such studies are too specific and normally employ a particular type of the assay only once. This prompts researchers to develop their own solutions for exploring herd immunity. In this work we discuss theoretical principles and practical approaches to developing multiplex screening assays and give examples of protocols and recommendations based on our own experience in the field.
Keywords: humoral immunity, immunological memory, vaccine-preventable infections, measles, mumps, rubella, hepatitis B, serologic diagnosis, multiplex immunoassay