ОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕ
Исследование клонального репертуара фракции активированных Т-лимфоцитов у пациента с анкилозирующим спондилитом
Российский национальный исследовательский медицинский университет имени Н. И. Пирогова, Москва, Россия
Для корреспонденции: Иван Владимирович Звягин
ул. Островитянова, д. 1, г. Москва, 117997; moc.liamg@nigayvzi
Финансирование: работа поддержана Министерством образования и науки РФ, идентификатор проекта RFMEFI60716X0158.
Благодарности: авторы выражают признательность пациенту, принявшему участие в исследовании; врачу-гематологу, профессору кафедры гематологии и терапии им. А. А. Максимова Денису Анатольевичу Федоренко из Национального медико-хирургического центра имени Н. И. Пирогова — за консультации по клиническим вопросам; старшему научному сотруднику Елене Ивановне Коваленко из Института биоорганической химии им. М. М. Шемякина и Ю. А. Овчинникова за помощь в осуществлении цитофлуорометрического анализа.
- Brown MA, Kenna T, Wordsworth BP. Genetics of ankylosing spondylitis — insights into pathogenesis. Nat Rev Rheumatol. 2016 Feb; 12 (2): 81–91. DOI: 10.1038/nrrheum.2015.133.
- Benjamin R, Parham P. Guilt by association: HLA-B27 and ankylosing spondylitis. Immunol Today. 1990 Apr; 11 (4): 137–42.
- Faham M, Carlton V, Moorhead M, Zheng J, Klinger M, Pepin F et al. Discovery of T-Cell Receptor Beta Motifs Specific to HLA-B27(+) Ankylosing Spondylitis by Deep Repertoire Sequence Analysis. Arthritis Rheumatol. 2017 Apr; 69 (4): 774–84. DOI: 10.1002/art.40028.
- Komech EA, Pogorelyy MV, Egorov ES, Britanova OV, Rebrikov DV, Bochkova AG et al. CD8+ T cells with characteristic TCR beta motif are detected in blood and expanded in synovial fluid of ankylosing spondylitis patients. Rheumatology (Oxford). Forthcoming 2018.
- Duchmann R, May E, Ackermann B, Goergen B, Meyer zum Büschenfelde KH, Märker-Hermann E. HLA-B27-restricted cytotoxic T lymphocyte responses to arthritogenic enterobacteria or self-antigens are dominated by closely related TCRBV gene segments. A study in patients with reactive arthritis. Scand J Immunol. 1996 Jan; 43 (1): 101–8.
- Dulphy N, Peyrat MA, Tieng V, Douay C, Rabian C, Tamouza R et al. Common intra-articular T cell expansions in patients with reactive arthritis: identical beta-chain junctional sequences and cytotoxicity toward HLA-B27. J Immunol. 1999 Apr 1; 162 (7): 3830–9.
- May E, Dulphy N, Frauendorf E, Duchmann R, Bowness P, Lopez de Castro JA et al. Conserved TCR beta chain usage in reactive arthritis; evidence for selection by a putative HLA-B27-associated autoantigen. Tissue Antigens. 2002 Oct; 60 (4): 299–308.
- Kivioja T, Vähärautio A, Karlsson K, Bonke M, Enge M, Linnarsson S et al. Counting absolute numbers of molecules using unique molecular identifiers. Nat Methods. 2011 Nov 20; 9 (1): 72–4. DOI: 10.1038/nmeth.1778.
- Mamedov IZ, Britanova OV, Zvyagin IV, Turchaninova MA, Bolotin DA, Putintseva EV et al. Preparing unbiased T-cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling. Front Immunol. 2013; 4: 456. Published online 2013 Dec 23. DOI: 10.3389/fimmu.2013.00456.
- Zvyagin IV, Mamedov IZ, Tatarinova OV, Komech EA, Kurnikova EE, Boyakova EV et al. Tracking T-cell immune reconstitution after TCRαβ/CD19-depleted hematopoietic cells transplantation in children. Leukemia. 2017; (31): 1145–53. DOI: 10.1038/leu.2016.321.
- Shugay M, Britanova OV, Merzlyak EM, Turchaninova MA, Mamedov IZ, Tuganbaev TR et al. Towards error-free profiling of immune repertoires. Nat Methods. 2014 Jun; 11 (6): 653–5. DOI: 10.1038/nmeth.2960. Epub 2014 May 4.
- Bolotin DA, Poslavsky S, Mitrophanov I, Shugay M, Mamedov IZ, Putintseva EV et al. MiXCR: software for comprehensive adaptive immunity profiling. Nat Methods. 2015 May; 12 (5): 380–1. DOI: 10.1038/nmeth.3364.
- Britanova OV, Putintseva EV, Shugay M, Merzlyak EM, Turchaninova MA, Staroverov DB et al. Age-related decrease in TCR repertoire diversity measured with deep and normalized sequence profiling. J Immunol. 2014 Mar 15; 192 (6): 2689–98. DOI: 10.4049/jimmunol.1302064.
- R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna, Austria. 2014. Available from: http://www.r-project.org/.
- Bisset LR, Lung TL, Kaelin M, Ludwig E, Dubs RW. Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland. Eur J Haematol. 2004 Mar; 72 (3): 203–12. DOI: 10.1046/j.0902-4441.2003.00199.x.
- Egorov ES, Merzlyak EM, Shelenkov AA, Britanova OV, Sharonov GV, Staroverov DB et al. Quantitative Profiling of Immune Repertoires for Minor Lymphocyte Counts Using Unique Molecular Identifiers. J Immunol. 2015 Jun 15; 194 (12): 6155–63. DOI: 10.1049/jimmunol.1500215.
- Qi Q, Liu Y, Cheng Y, Glanville J, Zhang D, Lee JY et al. Diversity and clonal selection in the human T-cell repertoire. Proc Natl Acad Sci U S A. 2014 Sep 9; 111 (36): 13139–44. DOI: 10.1073/pnas.1409155111.
- Koning D, Costa AI, Hoof I, Miles JJ, Nanlohy NM, Ladell K et al. CD8+ TCR repertoire formation is guided primarily by the peptide component of the antigenic complex. J Immunol. 2013 Feb 1; 190 (3): 931–9. DOI: 10.4049/jimmunol.1202466.
- Birnbaum ME, Mendoza JL, Sethi DK, Dong S, Glanville J, Dobbins J et al. Deconstructing the Peptide-MHC Specificity of T Cell Recognition. Cell. 2014 May 22; 157 (5): 1073–87. DOI: 10.1016/j.cell.2014.03.047.
- Zvyagin IV, Pogorelyy MV, Ivanova ME, Komech EA, Shugay M, Bolotin DA et al. Distinctive properties of identical twins’ TCR repertoires revealed by high-throughput sequencing. Proc Natl Acad Sci U S A. 2014 Apr 22; 111 (16): 5980–5. DOI: 10.1073/pnas.1319389111.
- Elhanati Y, Murugan A, Callan CG, Mora T, Walczak AM. Quantifying selection in immune receptor repertoires. Proc Natl Acad Sci U S A. 2014 Jul 8; 111 (27): 9875–80. DOI: 10.1073/pnas.1409572111.
- Pogorelyy MV, Minervina AA, Chudakov DM, Mamedov IZ, Lebedev YB, Mora T et al. Method for identification of condition-associated public antigen receptor sequences. BioRxiv 195057. DOI: 10.1101/195057.
- Dulphy N, Peyrat MA, Tieng V, Douay C, Rabian C, Tamouza R et al. Common intra-articular T cell expansions in patients with reactive arthritis: identical beta-chain junctional sequences and cytotoxicity toward HLA-B27. J Immunol. 1999 Apr 1; 162 (7): 3830–39.
- Akondy RS, Monson ND, Miller JD, Edupuganti S, Teuwen D, Wu H et al. The yellow fever virus vaccine induces a broad and polyfunctional human memory CD8+ T cell response. J Immunol. 2009 Dec 15; 183 (12): 7919–30. DOI: 10.4049/jimmunol.08039003.
- Meditz AL, Haas MK, Folkvord JM, Melander K, Young R, McCarter M et al. HLA-DR+ CD38+ CD4+ T Lymphocytes Have Elevated CCR5 Expression and Produce the Majority of R5-Tropic HIV-1 RNA In Vivo. J Virol. 2011 Oct; 85 (19): 10189–200. DOI: 10.1128/JVI.02529-10. Epub 2011 Aug 3.
- Blom K, Braun M, Ivarsson MA, Gonzalez VD, Falconer K, Moll M et al. Temporal dynamics of the primary human T cell response to yellow fever virus 17D as it matures from an effector- to a memory-type response. J Immunol. 2013 Mar 1; 190 (5): 2150–8. DOI: 10.4049/jimmunol.1202234.
- Blom K, Braun M, Pakalniene J, Dailidyte L, Béziat V, Lampen MH et al. Specificity and dynamics of effector and memory CD8 T cell responses in human tick-borne encephalitis virus infection. PLoS Pathog. 2015 Jan 22; 11 (1): e1004622. DOI: 10.1371/journal.ppat.1004622.
- Funderburg NT, Stubblefield Park SR, Sung HC, Hardy G, Clagett B, Ignatz-Hoover J et al. Circulating CD4(+) and CD8(+) T cells are activated in inflammatory bowel disease and are associated with plasma markers of inflammation. Immunology. 2013 Sep; 140 (1): 87–97. DOI: 10.1111/imm.12114.
- Dulic S, Vasarhelyi Z, Bajnok A, Szalay B, Toldi G, Kovacs L et al. The Impact of Anti-TNF Therapy on CD4+ and CD8+ Cell Subsets in Ankylosing Spondylitis. Pathobiology. 2017 Dec 6. DOI: 10.1159/000484250. [Epub ahead of print.]
- Shugay M, Bagaev DV, Zvyagin IV, Vroomans RM, Crawford JC, Dolton G et al. VDJdb: a curated database of T-cell receptor sequences with known antigen specificity. Nucleic Acids Res. 2018 Jan 4; 46 (D1): D419–D427. DOI: 10.1093/nar/gkx760.