ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Genetic characterization of Aerococcus sp. 1KP-2016 strain isolated from a patient with bloodstream infection
About authors
1 Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia
2 Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Andrey Viktorovich Chaplin
10 Admiral Makarov str., Moscow, 125212, Russia; oc.liamg@kidemoloko
About paper
Author contribution: AV Chaplin — phylogenetic analysis, data analysis, manuscript authoring; IA Chagina, AS Pimenova, NT Gadua — microbiological studies, manuscript authoring; NM Kargaltseva, LI Kafarskaya — literature analysis, manuscript authoring; OYu Borisova — molecular genetic studies, data analysis, literature analysis, manuscript authoring; EE Donskikh — data analysis, literature analysis, manuscript authoring.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Gabrichevsky Research Institute for Epidemiology and Microbiology (Minutes #28 of November 18, 2014); the patient signed a voluntary consent to participation in the study.
Received: 2023-03-22
Accepted: 2023-04-16
Published online: 2023-04-25
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Aerococcus genus bacteria are often associated with human urinary tract and bloodstream infections. The Aerococcus sp. 1KP-2016 strain isolated from the buffy coat had the 16S rRNA sequence that was a 98.7% (and less) match with the previously described members of this genus. The purpose of this study was to perform whole genome sequencing of Aerococcus 1KP-2016 followed by phylogenetic reconstruction. We have shown that Aerococcus 1KP-2016 belongs to the new species of the Aerococcus genus that is closest to Aerococcus viridans and Aerococcus urinaeequi. The genomic sequence, which consists of 2.042 million bps with GC content at 38.5%, was deposited in the DBJ/EMBL/GenBank under identifier NEEY00000000.
Keywords: whole genome sequencing, phylogenetic analysis, Aerococcus, bloodstream infection, buffy coat (blood leukocyte layer)