ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2017 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
METHOD
Universal method of staining of the infl ammatory centers and the intact brain tissues in vibratomic and semithin sections
About authors
1 Laboratory of Regulating Reparative Processes, Research Institute of General Pathology and Pathophysiology of RAMS, Moscow
2 Department of Hystology, Embryology and Cytology, Pediatric Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Ivan Dubrovin
ul. Baltiyskaya, d. 8, Moscow, Russia, 125315; ur.liam@prrl
Received: 2011-12-09
Accepted: 2012-09-19
Published online: 2017-01-05
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It is suggested a new method of brain tissue staining which contrasts cell parts like nucleus, cytoplasm, chromatin ultrastructure and the border of neuron cytoplasm, indistinguished by other methods. Equally with nervous tissue connective tissue is stained well also, blood vessels in particular. Macrophages become metachromatic. The proposed formular is universal and it is suitable for almost all objects of the study in the light microscope: paraffin, cryotomic, vibratomic and semithin sections.
Keywords: brain, inflammatory, centres, sections staining