METHOD

Universal method of staining of the infl ammatory centers and the intact brain tissues in vibratomic and semithin sections

Dubrovin IP1, Komissarova SV1, Turygina SA2
About authors

1 Laboratory of Regulating Reparative Processes, Research Institute of General Pathology and Pathophysiology of RAMS, Moscow

2 Department of Hystology, Embryology and Cytology, Pediatric Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Ivan Dubrovin
ul. Baltiyskaya, d. 8, Moscow, Russia, 125315; ur.liam@prrl

Received: 2011-12-09 Accepted: 2012-09-19 Published online: 2017-01-05
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It is suggested a new method of brain tissue staining which contrasts cell parts like nucleus, cytoplasm, chromatin ultrastructure and the border of neuron cytoplasm, indistinguished by other methods. Equally with nervous tissue connective tissue is stained well also, blood vessels in particular. Macrophages become metachromatic. The proposed formular is universal and it is suitable for almost all objects of the study in the light microscope: paraffin, cryotomic, vibratomic and semithin sections.

Keywords: brain, inflammatory, centres, sections staining

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