The aim of the study was to estimate the interconnection between cardiopulmonary exercise testing data and hormonal status of athletes. Adolescent and young adult winter sports athletes (n = 86), including biathlonists, skiers, ice-hockey players, snowboarders with middle age of 20,8 years old were examined. The cardiopulmonary exercise testing (MasterScreen Pneumo, Jaeger, Germany) was performed in a traditional 8-minute treadmill-test. Thyroid stimulating hormone, free thyroxin, cortisol, testosterone were examined in venous blood samples. The cardiopulmonary testing data, which characterize fitness, were significantly higher in males. In athletes, who did not reach the anaerobic threshold (AT), testosterone level was twice higher, than in those with estimated AT. The results of multiple stepwise regression analysis showed, that testosterone was the only one index, which influenced on the ergo-spirometry data distribution and associated with exercise performance in athletes.