New Approaches for Screening of Cardiac Troponin I in Plasma

About authors

1 Laboratory of Bioelectrochemistry, Department of Personalized Medicine,
V.N.Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

2 Department of Biochemistry, Biomedical Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Lyubov E. Agafonova
ul. Pogodinskaya, d. 10, str. 8, Moscow, Russia, 119121; ur.liam@abulavonofaga

Received: 2013-03-22 Accepted: 2013-04-25 Published online: 2017-01-05

The aim of the study was to develop new approaches for rapid and sensitive screening of cardiac troponin I in plasma. In this paper we developed a gravimetric and electrochemical immunosensors with experimental detection limits of cardiac troponin I in plasma: 0.4 ng/mL (17 pM) and 0.1 ng/mL (4 pM), which corresponds to the physiological level of cardiac marker in plasma for the diagnosis of acute myocardial infarction. The kinetics of highly specific interactions between the monoclonal antibody of cardiac troponin I and cardiac troponin I were also investigated by quartz crystal microbalance in real time, without multiple labeling procedures and multi-step process of immobilization of antibodies on a gold electrode, and secondary antibody staining.

Keywords: myocardial infarction, cardiac troponin I, cardiac marker, immunosensor, gold nanoparticles, stripping voltammetry method, quartz crystal microbalance method