ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2017 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
METHOD
Chemiluminescent determination of total antioxidant capacity in medicinal plant material
About authors
1 Department of Medical Biophysics, Faculty of Fundamental Medicine,Lomonosov Moscow State University, Moscow, Russia
2 Department of Pharmacognosy, Faculty of Pharmacy,The First Sechenov Moscow State Medical University, Moscow, Russia
Correspondence should be addressed: Georgiy Vladimirov
Lomonosovskiy prospekt, d. 31, k. 5, Moscow, Russia, 119192; ur.xednay@vorimidalv-aru
About paper
Funding: this work was supported by the Russian Science Foundation, grant no. 14-15-00375.
Acknowledgments: authors thank Andrey Alekseev from Lomonosov Moscow State University for his assistance in conducting the experiment.
Received: 2016-03-10
Accepted: 2016-03-18
Published online: 2017-01-05
|
Fig. 1. Effect of sodium ascorbate on chemiluminescence kinetics
Concentrations of components of the system: luminol — 40 μm, horseradish peroxidase — 4 nM, hydrogen peroxide — 100 μm. Curves: 1 — control sample; 2 — 0.05 μm; 3 — 0.10 μm; 4 — 0.15 μm; 5 — 0.2 μm; 6 — 0.25 μmM sodium ascorbate.
Fig. 2. Effect of tocopherol on chemiluminescence kinetics
Concentrations of components of the system: luminol — 40 μm, horseradish peroxidase — 4 nM, hydrogen peroxide — 100 μm. Curves: 1 — control sample; 2 — 0.01 μm; 3 — 0.025 μm; 4 — 0.06 μm; 5 — 0.1 μm; 6 — 0.2 μm of tocopherol.
Fig. 3. Effect of quercetin on chemiluminescence kinetics
Concentrations of components of the system: luminol — 40 μm, horseradish peroxidase — 4 nM, hydrogen peroxide — 100 μm. Curves: 1 — control sample; 2 — 0.02 μm; 3 — 0.03 μm; 4 — 0.04 μm; 5 — 0.05 μm; 6 — 0.06 μm of quercetin.
Fig. 4. Effect of fruit decoctions of mountain-ash (A), hawthorn (B), rose (C), and raspberry fruit infusion (D) on chemiluminescence kinetics
Concentrations of components of the system: luminol — 40 μm, horseradish peroxidase — 4 nM, hydrogen peroxide — 100 μm. (A) Curves: 1 — control sample; 2 — 0.002 g/L; 3 — 0.004 g/L; 4 — 0.006 g/L; 5 — 0.008 g/L of mountain-ash fruit decoction. (B) Curves: 1 — control sample; 2 — 0.005 g/L; 3 — 0.0075 g/L; 4 — 0.01 g/L; 5 — 0.0125 g/L of hawthorn fruit decoction. (C) Curves: 1 — control sample; 2 — 0.001 g/L; 3 — 0.0015 g/L; 4 — 0.002 g/L; 5 — 0.0025 g/L of rosehip decoction. (D) Curves: 1 — control sample; 2 — 0.001 g/L; 3 — 0.003 g/L; 4 — 0.004 g/L; 5 — 0.005 g/L of raspberry fruit infusion.
Content of antioxidants in the plant material according to chemical analysis and the total antioxidant capacity of the same objects, M ± δ
Note. * — literature data, [25–29]. ∆S — change in light sum for the sample, relative unit, C — sample concentration in the cuvette, g/L. The calculated values are significant at p <0.05.
