ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Wound care with the leaf extract of cecropin P1-producing transgenic kalanchoe: histological findings
About authors
1 Research Laboratory “Genetics”,
Kursk State University, Kursk, Russia
2 Kursk State Medical University, Kursk
3 Puschino branch of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry of RAS, Puschino, Russia
Correspondence should be addressed: Alexandr Belous
prospect A. Deriglazova, d. 71, kv. 89, Kursk, Russia, 305014; ur.liam@suoleb.s.a
About paper
Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (Grant No. 14.607.21.0016 dated June 5, 2014, ID RFMEFI60714X0016).
Acknowledgements: authors thank Viktor Lazarenko of Kursk State Medical University and Alexandr Khudin of Kursk State University for providing full access to the research facilities of both universities.
Contribution of the authors to this work: Belous AS, Zakharchenko NS — research planning, data collection; Shevelev AB — analysis of literature, data interpretation; Trubnikova EV — data interpretation, statistical analysis; Biryukova YuK — data interpretation, drafting a manuscript; Mishina ES, Loyko EA — data collection; Lebedeva AA — analysis of literature. All authors participated in editing of the manuscript.
Received: 2017-02-05
Accepted: 2017-02-18
Published online: 2017-03-14
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Fig. 1. Histological study of wounds modeled in rats. (A–C) Histological slices of animals in the control group (group 1) sacrificed at different stages of the experiment: (A) on day 3; (B) on day 10; (C) on day 14. (D–F) Histological slices of animals in group 2 (additional treatment with 10 % cefazolin) sacrificed at different stages of the experiment: (D) on day 3; (E) on day 10; (F) on day 14. Hematoxylin and eosin staining, ×280
Fig. 2. Histological study of wounds modeled in rats. (A–C) Histological slices of animals in group 3 (additional treatment with kalanchoe juice) sacrificed at different stages of the experiment: (A) on day 3; (B) on day 10; (C) on day 14. (D–F) Histological slices of animals in group 4 (additional treatment with kalanchoe juice containing antimicrobial peptide cecropin P1) sacrificed at different stages of the experiment: (D) on day 3; (E) on day 10; (F) on day 14. Hematoxylin and eosin staining, ×280
Proportion of various cells in the modeled wounds with regard to the treatment type and the day of sacrifice
