ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
siRNA-mediated gene silencing
About authors
1 Laboratory of Functional Genomics,Research Centre of Medical Genetics, Moscow, Russia
2 Laboratory of Medical and Genetic Technologies, Department of Basic Research of Research Institute for Medicine and Dentistry,Yevdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia
3 Genomic Functional Analysis Laboratory,Moscow Institute of Physics and Technology (State University), Dolgoprudny, Russia
Correspondence should be addressed: Mikhail Skoblov
ul. Moskvorechie, d. 1, Moscow, Russia, 115478; moc.liamg@volboksm
About paper
Contribution of the authors to this work: Vyakhirava JV — analysis of literature, research planning, data collection, analysis and interpretation, drafting of a manuscript; Filatova AYu — analysis of literature, data collection, analysis and interpretation, drafting of a manuscript; Krivosheeva IA — analysis of literature, data collection, analysis and interpretation, drafting of a manuscript; Skoblov MYu — drafting of a manuscript. All authors participated in editing of the manuscript.
Received: 2017-06-25
Accepted: 2017-06-28
Published online: 2017-07-19
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RNA-interference enjoys a wide range of applications in medical and biological research. In particular, it is used to study functions of genes. One of the most popular approaches to this task is gene knockdown by small interfering RNA (siRNA). Currently there is no unified protocol for this method, which results in low reproducibility of experimental data. In the following article we outline the theoretical bases for this method and provide practical recommendations for its use in siRNA-mediated gene silencing experiments.
Keywords: RNA interference, knockdown, gene expression