METHOD
The use of wild-type blocking allele-specific real-time polymerase chain reaction for the analysis of somatic mutations in RAS genes of circulating free DNA isolated from the blood plasma of patients with colorectal cancer
Laboratory of Molecular Biology and Cytogenetics,Russian Research Center of Roentgenoradiology, Moscow, Russia
Correspondence should be addressed: Galina Snigireva
ul. Profsoyuznaya, d. 86, Moscow, Russia, 117997; ur.liam@lag_ins
Acknowledgements: the authors thank Andrey Zaretsky of Evrogen (Moscow) for his help and valuable advice in conducting molecular genetic analysis.
Contribution of the authors to this work: Telysheva EN — analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript; Snigireva GP — research planning, data interpretation, drafting of a manuscript.