METHOD

The use of wild-type blocking allele-specific real-time polymerase chain reaction for the analysis of somatic mutations in RAS genes of circulating free DNA isolated from the blood plasma of patients with colorectal cancer

Telysheva EN, Snigireva GP
About authors

Laboratory of Molecular Biology and Cytogenetics,
Russian Research Center of Roentgenoradiology, Moscow, Russia

Correspondence should be addressed: Galina Snigireva
ul. Profsoyuznaya, d. 86, Moscow, Russia, 117997; ur.liam@lag_ins

About paper

Acknowledgements: the authors thank Andrey Zaretsky of Evrogen (Moscow) for his help and valuable advice in conducting molecular genetic analysis.

Contribution of the authors to this work: Telysheva EN — analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript; Snigireva GP — research planning, data interpretation, drafting of a manuscript.

Received: 2017-08-08 Accepted: 2017-08-17 Published online: 2017-10-29
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