ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Immunological memory formed in response to administration of GamTBvac recombinant tuberculosis vaccine candidate: clinical trials in healthy volunteers
About authors
1 Laboratory of Translational Biomedicine,N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow, Russia
2 Institute of Molecular Medicine,I. M. Sechenov First Moscow State Medical University, Moscow, Russia
3 Department of Virology, Faculty of Biology,Lomonosov Moscow State University, Moscow
Correspondence should be addressed: Denis A. Kleymenov
ul. Gamalei, d. 18, Moscow, Russia, 123098; ur.relbmar@tel00001
About paper
Funding: this work was supported by the Russian Ministry of Health and the Federal Agency for Scientific Organizations (state task no. 0574-2014-0023)
Contribution of the authors to this work: Kleymenov DA — analysis of literature, research planning, research preparation, data collection, analysis, and interpreatation, drafting of a manuscript, writing of a final version of a manuscript; Mazunina EP — analysis of literature, research planning, research preparation, data collection, analysis, and interpretation, drafting of a manuscript; Lunin VG — analysis of literature, research planning, research preparation, drafting of a manuscript; Koptev EYu, Manuilov VA — analysis of literature, research preparation, drafting of a manuscript; Gushchin VA — analysis of literature, research planning, research preparation, data analysis and interpretation, drafting of a manuscript, writing of a final version of a manuscript; Tkachuk AP — analysis of literature, research planning, research preparation, drafting of a manuscript, critical analysis of a manuscript.
Received: 2017-09-20
Accepted: 2017-10-20
Published online: 2018-01-13
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Fig. 1. The schematic of the study design. Stages 1 and 2 of the study reported in this article are shown in bold
Fig. 2. Immunogenicity of the GamTBvac vaccine in 12 volunteers. (A), (B) Evaluation of cell-mediated immunity. For the analysis we employed a modification of IGRA [19] that measures INFγ secretion by whole blood lymphocytes in response to stimulation with antigens DBD-Ag85A (А) and DBD-ESAT6-CFP10 (B). The value of spontaneous IFNγ secretion was subtracted from the raw value. (C)–(H) xMAP-based quantification of antibodies to the antigen fusion (C) and its components: CFP10 (D), ESAT6 (E), DBD (F), and DBD-Ag85A (G) and its main component Ag85A (H). Statistical significance was calculated using the Wilcoxon matched-pairs test. Significant differences over the observation period (*р < 0.05 and **р < 0.01) are shown in green, in comparison with day 0. Red p-values indicate the absence of significant differences. Vaccination days are represented by blue arrows
Concentrations of antigens used for coupling to microspheres
