ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2018 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Analysis of the apoptotic effect of ultrahigh gamma dose rates on human peripheral blood lymphocytes in vitro
About authors
1 Troitsk Institute for Innovation and Fusion Research (TRINITI),Rosatom State Atomic Energy Corporation, Troitsk, Moscow, Russia
2 Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia
3 Research Institute of Technical Physics and Automation,Rosatom State Atomic Energy Corporation, Moscow, Russia
4 Hertzen Moscow Cancer Research Institute (affiliated branch of the National Medical Research Radiology Center), Moscow, Russia
5 Russian Scientific Center of Roentgenoradiology, Moscow
Correspondence should be addressed: Vladimir K. Bojenko
ul. Profsoyuznaya, d. 86, Moscow, Russia, 117997; ur.liam@oknejobv
About paper
Funding: this work was supported by Rosatom and the Russian Science Foundation (Grant no. 15-10355).
All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.
Received: 2017-11-22
Accepted: 2017-12-05
Published online: 2018-01-25
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Fig. 1. Schematic of generation and delivery of the electromagnetic pulse by Angara-5-1 generator to the load in the vacuum chamber. PFL — double water pulse- forming line; TL — water transmission line; VWI — vacuum-water interface; VTL — magnetically insulated vacuum transmission line; VC — vacuum chamber with electromagnetic flux concentrator
Fig. 2. Position of the samples for irradiation by Mir-M (A, B), Angara-5-1 (C) and Rokus-AM (D)
Fig. 3. Levels of apoptosis in the suspensions of peripheral blood lymphocytes irradiated by Rokus-AM and Angara-5-1 with doses from 0 to 5 Gy
Fig. 4. Comparison of the effects induced by electron beams generated by high-current nanosecond electron accelerator Mir-M and gamma rays produced by Rokus-AM on human peripheral blood lymphocytes in the dose range from 0 to 6 Gy. (A) Early apoptosis (annexin V+/PI–); (B) late apoptosis (annexin V+/PI+); (C) necrosis (annexin V–/PI+); (D) total cell death. PI — propidium iodide. Positive and negative staining is represented by + and – respectively
