ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Analysis of the association between the RS767455 T>C TNFRSF1A and RS1061622 T>G TNFRSF1B polymorphisms and nonalcoholic steatohepatitis
About authors
1 Institute of Biology, Karelian Research Center of RAS, Petrozavodsk, Republic of Karelia
2 Institute of Medicine, Petrozavodsk State University, Petrozavodsk, Republic of Karelia
Correspondence should be addressed: Ludmila Topchieva
ul. Pushkinskaya 11, Petrozavodsk, 198910; ur.liam@76aveihcpot
About paper
Funding: this study was part of the public contract 0221-2017-0049 and was carried out using the equipment of the shared facility Complex Basic and Applied Research of Living Systems in the Arctic of the Institute of Biology, Karelian Research Center. The work was also sponsored by a scholarship of the President of the Russian Federation for young scientists and graduate students engaged in advanced research and development in priority areas of modernization of the Russian economy in 2015-2017 years. The authors also received support from Petrozavodsk State University as part of the efforts for its strategic development in 2013– 2017 (R&D 115070110006, Information reference map 216022450003, registered February 24, 2016) under the project for the Development of Technologies for Diagnostic Screening for Nonalcoholic Fatty Liver Disease in Overweight Patients and Patients with Metabolic Syndrome (ID 9173GU/2015 dated December 15, 2015) of the UMNIK program.
Received: 2017-10-31
Accepted: 2018-03-02
Published online: 2018-04-15
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Fig. 1. Electrophoresis of rs767455-containing PCR products after restriction
digest: М — Thermo Scientific GeneRuler Low range DNA Ladder, 1 — genotype
CС (330 bp), 2 — genotype TС (330, 184 and 146 bp), 3 — genotype TT (184
and 146 bp)
Fig. 2. Electrophoresis of rs1061622-containing PCR products after restriction
digest: М — Thermo Scientific GeneRuler Low range DNA Ladder, 1 — genotype
TT (235 and 144 bp),2 — genotype TG (379, 235 and 144 bp), 3 — genotype
GG (379 bp)
Table 1. Distribution of TNFRSF1A T>C (rs767455) alleles and genotypes in patients with NASH and healthy control
Table 2. Distribution of TNFRSF1B T>G (rs1061622) alleles and genotypes in patients with NASH and healthy controls
Table 3. Liver function parameters in healthy and diseased TNFRSF1B 587T>G (rs1061622) carriers
Note: data are represented as mean and error mean (M ± m). The median is shown in brackets.
