2018 / 02

Следующая статья
DOI: 10.24075/vrgmu.2018.021

ОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕ

Cas13a: получение и использование для определения вирусной РНК

А. С. Савинова1,2, Е. Ю. Коптев1, Е. В. Усачев1, А. П. Ткачук1, В. А. Гущин1,3
Информация об авторах

1 Национальный исследовательский центр эпидемиологии и микробиологии имени Н. Ф. Гамалеи, Москва

2 Лаборатория трансляционной биомедицины,
Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени Н. Ф. Гамалеи, Москва

3 Кафедра вирусологии, биологический факультет,
Московский государственный университет имени М. В. Ломоносова, Москва

Для корреспонденции: Владимир Алексеевич Гущин
ул. Гамалеи, д. 18, г. Москва, 123098; 89037515786; moc.liamg@adainawow; gro.ayelamag@nihchsug.a.rimidalv

Статья получена: 31.05.2018 Статья принята к печати: 07.06.2018 Опубликовано online: 01.07.2018
|
  1. Makarov VV, Khromov AV, Gushchin VA, Tkachuk AP. Emergence Of New Infections In The 21st Century And Identification Of Pathogens Using Next Generation Sequencing. Bulletin of Russian State Medical University. 2017; 01: 5–25.
  2. Fournier PE, Dubourg G, Raoult D. Clinical detection and characterization of bacterial pathogens in the genomics era. Genome medicine. 2014; 6 (11): 114.
  3. Karadeema RJ, Stancescu M, Steidl TP, Bertot SC, Kolpashchikov DM. The owl sensor: a 'fragile' DNA nanostructure for the analysis of single nucleotide variations. Nanoscale. 2018; 10: 10116–22.
  4. Durmanov NN, Guliev RR, Eremenko AV, Boginskaya IA, Ryzhikov IA, Trifonova EA, et al. Non-labeled selective virus detection with novel SERS-active porous silver nanofilms fabricated by Electron Beam Physical Vapor Deposition. Sensors Actuators B: Chemical. 2018; 257: 37–47.
  5. Pachauri V, Ingebrandt S. Biologically sensitive field-effect transistors: from ISFETs to NanoFETs. Essays Biochemistry. 2016; 60 (1): 81–90.
  6. Stein V, Alexandrov K. Synthetic protein switches: Design principles and applications. Trends in Biotechnology. 2015; 33 (2): 101–10.
  7. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018; 360 (6387): 439–44.
  8. East-Seletsky A, O’Connell MR, Knight SC, Burstein D, Cate JHD, Tjian R, et al. Two distinct RNase activities of CRISPRC2c2 enable guide-RNA processing and RNA detection. Nature. 2016; 538 (7624): 270–73.
  9. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, et al. Nucleic acid detection with CRISPR-Cas13a/ C2c2. Science. 2017; 356 (6336): 435–42.
  10. Liu L, Li X, Wang J, Wang M, Chen P, Yin M, et al. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. Cell. 2017; 168 (1–2): 121–34.
  11. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Analitical Biochemistry. 1989; 182 (2): 319–26.
  12. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Science. 1995; 4 (11): 2411–23.
  13. Li CX, Shi M, Tian JH, Lin XD, Kang YJ, Chen LJ, et al. Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. Elife. 2015; 4: 1–26.
  14. Shi M, Lin XD, Tian JH, Chen LJ, Chen X, Li CX, et al. Redefining the invertebrate RNA virosphere. Nature. 2016; 540 (7634): 539– 43.
  15. Gao GF. Commentary From “ A ” IV to “ Z ” IKV: Attacks from Emerging and Re-emerging Pathogens. Cell. 2018; 172 (6): 1157–59.