ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Human enteroviruses exhibit selective oncolytic activity in the model of human glioblastoma multiforme xenografts in immunodeficient mice
About authors
1 Engelhardt Institute of Molecular Biology, Moscow, Russia
2 Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow
3 Pirogov Russian National Research Medical University, Moscow, Russia
4 N. N. Blokhin Russian Cancer Research Center, Moscow, Russia
Correspondence should be addressed: Peter M. Chumakov
ul. Vavilova, 32, Moscow, 119991; moc.oohay@mpvokamuhc
About paper
Funding: the study was supported by the Ministry of Education and Science of the Russian Federation; project code RFMEFI60714X0014.
Received: 2018-06-26
Accepted: 2018-06-30
Published online: 2018-07-13
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Fig. 1. Sensitivity of early-passage monolayer cell cultures obtained from two glioblastoma patients to four strains oncolytic enteroviruses: —○— — Type 1 Poliovirus; --∆-- — Coxsackie virus A7; —□— — Coxsackie virus A9; ···●··· — Coxsackie virus B5. Horizontal scale — 10-8—10-2 — ten-fold serial dilutions of viral stocks used for the infections. Vero — control cell line that is sensitive to all four types of viruses used
Fig. 2. Tumorigenicity-inhibition assay with glioblastoma neurosheres pretreated with each f the four viruses before being injected subcutaneously into nude mice. Bars reflect number of tumors formed in each treated and untereated (Contr) group of mice. PVS1 — Type 1 Poliovirus; CV-A7 — Coxsackie virus A7; CV-A9 — Coxsackie virus A9; CV-B5 — Coxsackie virus B5
