2018 / 02

Next Paper
DOI: 10.24075/brsmu.2018.026
Copyright: © 2018 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Human enteroviruses exhibit selective oncolytic activity in the model of human glioblastoma multiforme xenografts in immunodeficient mice

Zheltukhin AO1, Soboleva AV1,2, Sosnovtseva AO1,3, Le TH1, Ilyinskaya GV1,4, Kochetkov DV1, Lipatova AV1, Chumakov PM1,2
About authors

1 Engelhardt Institute of Molecular Biology, Moscow, Russia

2 Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow

3 Pirogov Russian National Research Medical University, Moscow, Russia

4 N. N. Blokhin Russian Cancer Research Center, Moscow, Russia

Correspondence should be addressed: Peter M. Chumakov
ul. Vavilova, 32, Moscow, 119991; moc.oohay@mpvokamuhc

About paper

Funding: the study was supported by the Ministry of Education and Science of the Russian Federation; project code RFMEFI60714X0014.

Received: 2018-06-26 Accepted: 2018-06-30 Published online: 2018-07-13
|
Fig. 1. Sensitivity of early-passage monolayer cell cultures obtained from two glioblastoma patients to four strains oncolytic enteroviruses: —○— — Type 1 Poliovirus; --∆-- — Coxsackie virus A7; —□— — Coxsackie virus A9; ···●··· — Coxsackie virus B5. Horizontal scale — 10-8—10-2 — ten-fold serial dilutions of viral stocks used for the infections. Vero — control cell line that is sensitive to all four types of viruses used
Fig. 2. Tumorigenicity-inhibition assay with glioblastoma neurosheres pretreated with each f the four viruses before being injected subcutaneously into nude mice. Bars reflect number of tumors formed in each treated and untereated (Contr) group of mice. PVS1 — Type 1 Poliovirus; CV-A7 — Coxsackie virus A7; CV-A9 — Coxsackie virus A9; CV-B5 — Coxsackie virus B5