ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Chimeric antigen receptor expression in natural killer cell line NK-92 by transduction with lentiviral particles pseudotyped with the surface glycoproteins of the measles virus vaccine strain
About authors
Group of structural Organization of T-cell Immunity, Department of Adaptive Immunity Genomics, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow
Correspondence should be addressed: Stepan P. Chumakov
Miklouho-Maclay 16/10, Moscow, 117997;
moc.liamg@lukhtah
About paper
Funding: this work was funded by MESR (project code RFMEFI60716X0156).
Received: 2018-11-27
Accepted: 2018-12-20
Published online: 2018-12-31
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Fig. 1. The share of fluorescent HEK-293 cells, measured 48 hours post transduction by H/F-pseudotyped or VSV-G-pseudotyped lentiviral vectors. Axis Х — plasmid ratios for vector:psPAX2:pMD2-FΔ30:pCG-H
Fig. 2. Syncytia formed by HEK-293T cells after transfection with plasmid mixture for production of H/F-pseudotyped lentivirus particles. А. pCG-HcΔ18 + pMD2-FΔ30. B. pCG-HcΔ24 + pMD2-FΔ30. C. pCG-4A-HcΔ24 + pMD2-FΔ30. D. pMD2-G
Fig. 3. Proliferative activity of NK-92 cells, measured 48 hours post addition of different amounts of BX795 to the cultivation media. All values were normalized relative
to control (untreated culture). Values below 0.5 are characteristic to the culture that wasn’t proliferating after addition of BX795
Fig. 4. Suppression of proliferation of Raji cells upon co-culturing with NK-92 cells. Axis Y — % of Raji with normal phenotype (FSC/SSC) after 2 days of co-culturing,
compared to control sample (no NK-92 addition). Series: non-transduced NK-92; NK-92, transduced by tagRFP expressor (non-selected); NK-92, transduced by CAR
@CD20 after selection on magnetic microspheres
Table. Infectious viral titers of viral stocks, produceв with different variants of
H protein. All values were calculated per 106 packaging cells
