2019 / 01

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DOI: 10.24075/brsmu.2019.005

OPINION

Glycoprotein GP as a basis for the universal vaccine against Ebola virus disease

Dolzhikova IV, Tukhvatulin AI, Gromova AS, Grousova DM, Tukhvatulina NM, Tokarskaya EA, Logunov DYu, Naroditskiy BS, Gintsburg AL
About authors

Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia

Correspondence should be addressed: Inna V. Dolzhikova
Gamalei 18, Moscow, 123098; moc.liamg@avokihzlod.i

About paper

Author contribution: Dolzhikova IV, Tukhvatulin AI and Logunov DY conceived and planned the study, analyzed the literature, collected, analyzed and interpreted the data; Gromova AS, Grousova DM, Tukhvatulina NM, and Tokarskaya EA helped to collect and analyze the data; Naroditskiy BS and Gintsburg AL contributed to data interpretation; Dolzhikova IV wrote this manuscript.

Received: 2018-12-06 Accepted: 2019-02-20 Published online: 2019-03-03
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Fig. 1. The structure of Ebolavirus. GP — glycoprotein; L — catalytic subunit of the viral RNA-dependent RNA polymerase; NP — nucleoprotein; VP24 — minor matrix protein; VP30 — minor nucleoprotein; VP35 — nucleocapsid protein; VP40 — matrix protein
Fig. 2. Forms of Ebolavirus glycoprotein in eukaryotic cells. GC — glycan cap (glycan cap); HR — heptad repeat; HPR — hydrophobic region; MLD — mucin-like domain (mucin domain); RBD — receptor-binding domain; SP — signal peptide; TD — transmembrane domain
Fig. 3. The aligned consensus amino acid sequences of glycoprotein of ZEBOV, SUDV and BDBV species. Key amino acid positions essential for antibody binding are marked by a rectangle [34, 36]
Table 1. EVD case fatality in patients infected with different ebolaviruses. The table shows summarized data on the total number individuals with EVD in all reported outbreaks [4]
Note: * — clinical symptoms not observed; antibodies against RESTV detected in the blood serum.
Table 2. Homology of amino acid sequences of glycoprotein IE in ZEBOV, SUDV and BDBV viruses. The search for IE was conducted in T Cell Epitope Prediction Tools [32]; amino acid sequences were compared in Geneious® 10.2.3 (Biomatters; Auckland, New Zealand). The heat map shows homology is expressed as %: dark gray stands for 100%, white represents 0%
Note: * — IE essential for inducing protective T-cell-mediated immune response [22]; ** — IE essential for inducing protective B-cell-mediated immune response [20, 34–38].
Table 3. Homology of amino acid sequences of ZEBOV glycoprotein IE (isolates from 1976, 1995, 2014 and 2018). The search for IE was conducted in T Cell Epitope Prediction Tools [32]; amino acid sequences were compared in Geneious® 10.2.3 (Biomatters; Auckland, New Zealand). The heat map shows homology is expressed as %: dark gray stands for 100%, white represents 0%
Note: * — IE essential for inducing protective T-cell-mediated immune response [22]; ** — IE essential for inducing protective B-cell-mediated immune response [20, 34–38].