Bulletin of RSMU

ORIGINAL RESEARCH

Detection of Ser450Leu mutation in rpoB gene of Mycobacterium tuberculosis by allele-specific loop-mediated isothermal DNA amplification method

Filipenko ML¹, Oscorbin IP¹, Khrapov EA¹, Shamovskaya DA¹, Cherednichenko AG², Shvartz YaSh²

¹ Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia

² Novosibirsk Tuberculosis Research Institute, Novosibirsk, Russia

Correspondence should be addressed: Maxim L. Filipenko
Lavrentyev Prospect 8/2, Novosibirsk, 630090; moc.liamg@oknepiliflm

About paper

Funding: the study was done with the financial support of the basic budgetary financing project № VI.62.1.5 «Synthetic biology: development of tools for the genetic material manipulation and creation of promising drugs for therapy and diagnostics» (0309-2018-0003).

Author contribution: Filipenko ML created а general concept of the study, planned experiments, analyzed the results and participated in the writing of this article; Oscorbin IP participated in the writing of this article; Khrapov EA conducted experiments; Shamovskaya DV conducted experiments; Cherednichenko AG analyzed the experiments results; Shvartz YaSh was involved in planning and analyzed the experiments results.

Received: 2018-12-07 Accepted: 2019-02-25 Published online: 2019-03-09

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ORIGINAL RESEARCH Detection of Ser450Leu mutation in rpoB gene of Mycobacterium tuberculosis by allele-specific loop-mediated isothermal DNA amplification method

Filipenko ML, Oscorbin IP, Khrapov EA, Shamovskaya DA, Cherednichenko AG, Shvartz YaSh

Published in "Bulletin of RSMU" 1, 2019

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Vancouver style

Filipenko ML, Oscorbin IP, Khrapov EA, Shamovskaya DA, Cherednichenko AG, Shvartz YS. Detection of Ser450Leu mutation in rpoB gene of Mycobacterium tuberculosis by allele-specific loop-mediated isothermal DNA amplification method. Bulletin of RSMU. 2019; (1): 34–40. DOI: 10.24075/brsmu.2019.007

Harvard style

Filipenko, M. L., Oscorbin, I. P., Khrapov, E. A., Shamovskaya, D. A., Cherednichenko, A. G. and Shvartz, Y. S. (2019), "Detection of Ser450Leu mutation in rpoB gene of Mycobacterium tuberculosis by allele-specific loop-mediated isothermal DNA amplification method", Bulletin of RSMU, no. 1, p. 34–40. DOI: 10.24075/brsmu.2019.007

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ORIGINAL RESEARCH

Detection of Ser450Leu mutation in rpoB gene of Mycobacterium tuberculosis by allele-specific loop-mediated isothermal DNA amplification method