Space medicine has long studied the impact of reduced gravity on the human body. Increasing complaints of insufficient visual acuity during and after space flights have been recently drawing a lot of attention to the effects of hypogravity on astronauts’ vision. Abnormally high intraocular pressure (IOP) is one of the most clinically important changes occurring during space missions. It is a serious condition that often causes irreversible damage to the optic nerve and blindness. The aim of this study was to explore the effect of reduced gravity on IOP. The study recruited 48 young healthy men with the mean age of 22 years, who formed 2 equally sized groups. In the experimental group, hypogravity was simulated by placing the subjects into the orthostatic position for 21 days. IOP was measured at 4 time points using a Maklakov tonometer. Z-approximation of the Wilcoxon T test was applied. The average increase in IOP in the experimental group was 3.42 ± 0.03 mmHg (р < 0.01). The changes were, however, transient, and IOP levels went back to normal right after the exposure to hypogravity conditions was terminated.