Quantitative PCR in diagnosing infectious urogenital pathology

Rakhmatulina MR1, Galkina IS2
About authors

1 A. I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia

2 Federal Research Institute for Health Organization and Informatics, Moscow, Russia

Correspondence should be addressed: Margarita R. Rakhmatulina
Novoyasenevsky Prospekt, 9, Moscow, 117588; ur.xednay@gram.ar

About paper

Declarations: the authors declare a conflict of interests in connection with affiliation with DNA-Technology JSC, developer and manufacturer of the reagents mentioned in the article.

Author contribution: Rakhmatulina MR — literature data analysis, text authoring and editing; Galkina IS — literature data analysis, text authoring.

Received: 2019-12-03 Accepted: 2019-12-18 Published online: 2019-12-25

This article describes the contemporary methods of diagnosing sexually transmitted infections, their advantages and disadvantages, indications for use. The authors describe application of quantitative polymerase chain reaction in diagnosing inflammatory diseases and dysbiotic conditions in men and women. This method, which is currently the “golden standard” in urogenital pathology diagnostics, has undeniable advantages over microbiological methods and qualitative polymerase chain reaction: the preanalytical stage requirements (preservation of quantitative ratios between microorganisms or nucleic acids of microorganisms) are not as strict, the risk of contamination from outside environment and subsequent corruption of the results is significantly smaller, the conditions for all microorganisms, including those impossible and hard to cultivate, are the same sensitivity and specificity-wise, it is possible to sample materials and evaluate microbiota (ratios of microorganisms and their groups) and also possible to collect samples non-invasively, the speed of testing is high.

Keywords: qPCR, microbial biocenosis, urogenital infections