2020 / 02

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DOI: 10.24075/brsmu.2020.017

ORIGINAL RESEARCH

Circadian rhythms of leukemia inhibitory factor in the blood of patients with essential hypertension

Radaeva OA1, Simbirtsev AS2, Gromova EV1, Iskandiarova MS1, Belyaeva SV3
About authors

1 Institute of Medicine, National Research Mordovia State University, Saransk, Russia

2 State Research Institute of Highly Pure Biopreparations, FMBA, St. Petersburg, Russia

3 North Caucasus Health Center, Pyatigorsk, Russia

Correspondence should be addressed: Olga A. Radaeva
Ulianova, 26a, Saransk, 430000; ur.liam@97_fybwbltv

About paper

Compliance with ethical standards: the study was approved by the Ethics Committee of National Research Mordovia State University (Protocol No. 12 dated December 14, 2008). Written informed consent was obtained from all study participants. Blood samples were collected in compliance with the Declaration of Helsinki (2008), the protocol of European Convention on Human Rights and Biomedicine (1999) and the additional protocol to the Convention on Human Rights and Biomedicine concerning Biomedical Research (2005).

Author contribution: Radaeva OA designed the study, analyzed the results, formulated the conclusions and wrote the manuscript; Simbirtsev AS formulated the objective of the study, revised its conclusions and the manuscript itself; Gromova EV designed the study, carried out laboratory tests, and contributed to writing the manuscript; Iskandiarova MS analyzed the literature, supervised blood collection, followed up with the patients, contributed to writing the manuscript; Belyaeva SV analyzed the literature, supervised blood collection, followed up with the patients.

Received: 2020-03-11 Accepted: 2020-03-25 Published online: 2020-03-29
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Table 1. LIF concentrations (pg/ml) in the peripheral blood serum in patients with stage II EH at 8:00, 14:00, 20:00, 2:00, and 8:00 o’clock (next day) in the absence/ presence of antihypertensive therapy in patients with or without cardiovascular complications developed in the 5-year follow-up period (Ме (Q25%–Q75%))
Note: significant for comparisons with the specified time of blood collection or the group (a, b, c, c 1, c2); * — p < 0.001; ^ — p < 0.01; ' — p < 0.05. The paired t-test was applied for intragroup comparison of pretreatment results obtained at 8:00, 14:00, 20:00, 2:00 and 8:00 o’clock. The Wilcoxon test was applied for intragroup comparison in the group of patients after one year of treatment and in the healthy controls. The Mann–Whitney U test was applied to compare independent samples. The Wilcoxon test was applied to compare dependent samples.
Table 2. The percentage of deviations of blood serum LIF (pg/ml) measured at 14.00, 20.00, 2.00 and 8.00 o’clock (next day) from the initial concentrations measured at 8:00 of the first day in patients with stage II EH in the absence/presence of antihypertensive therapy with or without cardiovascular complications developed in the 5-year follow-up period (Ме (Q25%–Q75%))
Note: significant for comparisons with the specified time of blood collection or the group (a, b, c, c1, c2). * — p < 0.001; ^ — p < 0.01; ' — p < 0.05. The paired t-test was applied for intragroup comparison of pretreatment results obtained at 8:00, 14:00, 20:00, 2:00 and 8:00 o’clock. The Wilcoxon test was applied for intragroup comparison in the group of patients after one year of treatment and in the healthy controls. The Mann-Whitney U test was applied to compare independent samples. The Wilcoxon test was applied to compare dependent samples.