ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
New in vitro model to evaluate kinetics of antimycobacterial drug release from bioresorbable polymeric carriers
About authors
1 Laboratory of Biotechnology, Central Tuberculosis Research Institute, Moscow, Russia
2 Institute of Photon Technologies FSRC ‘Crystallography and Photonics’ RAS, Moscow, Russia
Correspondence should be addressed: Sofia N. Andreevskaya
Yauzskaya alleya, d. 2, str. 1A., Moscow, 107564; ur.liam@aifosdna
About paper
Funding: the study was supported by the Russian Ministry of Science and Higher Education and carried out as part of the State Assignment for FSRC “Crystallography and Photonics” RAS (developing an SCF-based method for creating bioactive matrices), as part of the State Assignment № 0515-2019-0015 (Formation of resistance to antimycobacterial drugs in mycobacteria and somatic cells) for the Central Tuberculosis Research Institute (evaluation of the bacteriostatic activity of different levofloxacin concentrations). The study was also supported by the Russian Foundation for Basic Research (Project № 18-29-06062 mk: development of sustained-release therapeutic formulations and an in vitro model for the evaluation of their efficacy).
Compliance with ethical standards: manipulations with virulent strains of M. tuberculosis were conducted in compliance with the safety guidelines for the experiments involving Risk Group III–IV pathogens, infectious agents and utilization of medical waste specified in sanitary regulations 1.3.2322-08, 1.3.2518-09, 1.3.2885-11, and 2.1.7.2790-10.
Author contribution: Andreevskaya SN — interpretation of study results, manuscript preparation; Smirnova TG — modeling of conditions for levofloxacin release from its carriers; discussion of study results; Antonov EN — preparation of matrices; discussion of study results; Chernousova LN, Ergeshov AE — study design; discussion of study results; Bogorodsky SE — preparation of microparticles; discussion of study results; Larionova EE — literature analysis; discussion of study results; Popov VK — method for antibiotic encapsulation into polymeric carriers; discussion of study results.
Received: 2020-08-06
Accepted: 2020-08-20
Published online: 2020-08-30
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Figure. An LFX dose-response curve for the susceptible H37Rv strain of M. tuberculosis
Table 1. Growth dynamics of M. tuberculosis H37Rv in the presence of levofloxacin
Table 2. Growth inhibition of M. tuberculosis H37Rv exposed to LFX released from a polymeric carrier
Note: * — calculated based on the growth delay relative to the control drug-free culture; ** — determined from the dose-response curve (see Figure); *** — hereinafter (in the experiments involving matrix washing): accounts for the drug amount washed from the carrier surface (% of the washed formulation = % of the unwashed drug released into the medium within 3 h); unwashed — indicates the experiment without carrier washing, washed – indicates the experiment involving carrier washing; – — the amount of released LFX is not enough to achieve the desired bacteriostatic effect (< 0.125 μg/ml).
