ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
The importance of determining SARS-CoV-2 N-Ag serodiagnostics for the management of COVID-19 pneumonia in hospital settings
About authors
1 XEMA Co. Ltd., Moscow, Russia
2 Federal Center for Brain Research and Neurotechnologies of FMBA, Moscow, Russia
3 Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Moscow, Russia
About paper
Funding: this work was partially supported by a grant № 075-15-2019-1789 from the Ministry of Science and Higher Education of the Russian Federation allocated to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine.
Acknowledgement: we acknowledge Natalia Usman for helpful discussions
Author contribution: Lebedin YS designed the study, performed the sampling, conducted molecular studies and drafted the manuscript, Lyang OV and Galstyan AG performed the clinical examinations and sampling, conducted biochemical and molecular studies, Panteleeva AV conducted molecular studies and drafted the manuscript, Belousov VV and Rebrikov DV designed the study and drafted the manuscript. All authors read and approved the final version of the manuscript.
Compliance with ethical standards: the study protocol was reviewed and approved by the Local Ethics Committee at the Pirogov Russian State Medical University (Protocol № 2020/07 dated March 16, 2020,); the study was conducted in accordance with the Declaration of Helsinki.
Received: 2021-02-11
Accepted: 2021-02-24
Published online: 2021-02-28
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Fig. 1. Dynamics of serum N-Ag levels in the studied cohort of hospital patients. ULQ, upper limit of quantification. The 'PCR+' and 'PCR-' indications refer to the patient's status on admission
Fig. 2. Dynamics of serum N-Ag levels vs. respective IgG antibodies in patients IgG-seropositive on admission (A) and patients IgG-seronegative on admission (B). Vertical axes correspond to Ag concentration (on the left) and Ab index (on the right).ULQ, upper limit of quantification; LOD, (lower) limit of detection
Fig. 3. Dynamics of serum N-Ag levels vs. respective IgM antibodies in patients IgM-seropositive on admission (A) and patients IgM-seronegative on admission (B). Vertical axes correspond to Ag concentration (on the left) and Ab index (on the right).ULQ, upper limit of quantification; LOD, (lower) limit of detection
Fig. 4. Individual patterns of seroconversion in the course of 3–5 days after the admission. ULQ, upper limit of quantification; LOD, (lower) limit of detection; PT, positivity threshold
