ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Evaluation of SARS-CoV-2 viability on experimental surfaces over time
About authors
1 Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia
2 Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
3 Lomonosov Moscow State University, Moscow, Russia
Correspondence should be addressed: Maria A. Nikiforova
Gamaleya, 18, Moscow, 123098; ur.xobni@avorofikinairam
About paper
Funding: this research was funded by the grant #056 - 00119 - 21-00 provided by the Ministry of Health of the Russian Federation, Russia.
Acknowledgments: the authors are grateful to Dr. I.V. Korobko for the general idea and discussion of the study design.
Author contribution: Nikiforova MA — experiment planning, working with the virus and determining SARS-CoV-2 viability, data analyzing, writing-original draft preparation; Siniavin AE — working with the virus and determining SARS-CoV-2 viability, data analysing, writing-original draft preparation; Shidlovskaya EV — PCRanalysis, data processing, writing-original draft preparation; Kuznetsova NA — PCR-analysis; Gushchin VA — experiment planning, writing-original draft preparation.
Received: 2021-06-25
Accepted: 2021-07-09
Published online: 2021-07-13
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Infected SARS-CoV-2 virus occurs not only through contact with an infected person, but also through surfaces with wich the illnes has contacted. The problem of preserving an infectious virus over time capable of infecting remains actual. We evaluated the SARS-CoV-2 viability preservation on different model surfaces over time. Ceramic tiles, metal (aluminum foil), wood (chipboard), plastic and cloth (towel) were used as model materials. Assessment of the presence of SARS-CoV-2 RNA was carried out by quantitative RT-PCR. Viable virus was determined by tissue culture assay on 293T/ACE2 cells. It was found that the SARS-CoV-2 RNA was detected on all studied surfaces for 360 minutes, but a significant decrease RNA by 1 log10 copies/ml was detected after contact of the virus with cloth (towel). While the viability of the virus was completely lost after 120 minutes. Type of experimental surface significantly affects viability preservation.
Keywords: coronavirus, PCR, SARS-CoV-2, viability, surface