ORIGINAL RESEARCH

Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

About authors

1 Kursk State Medical University, Kursk, Russia

2 Institute of Molecular Genetics, RAS, Moscow, Russia

Correspondence should be addressed: Anton O. Vorvul
Karl Marx str. 3, Kursk, 305041, Russia; ur.liam@6991luvrov

About paper

Author contribution: Vorvul AO — performing experiments, quantification of oxidative stress markers, data acquisition and statistical processing, manuscript writing; Bobyntsev II — study concept and design, manuscript writing; Medvedeva OA — study concept and design; Azarova YuE — quantification of oxidative stress markers; Belykh AE — manuscript writing; Andreeva LA — study concept and design, peptide synthesis.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Kursk State Medical University (protocol № 3 dated November 16, 2020). All the experiments were in line with the ARRIVE guidelines and were performed in accordance with the Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes.

Received: 2021-10-28 Accepted: 2021-11-14 Published online: 2021-11-28
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Fig. 1. Study design
Fig. 2. Effects of the ACTH(6-9)-Pro-Gly-Pro peptide on the intensity of free-radical processes and stress response intensity against the background of CRS. * — p < 0.05, significance level of the differences compared with the control group not subjected to stress (based on Welch's t-test); # — p < 0.05, significance level of the differences compared with the control group (based on Mann–Whitney U-test); $ — p < 0.05, significance level of the differences compared with the control group subjected to stress (based on one-way ANOVA with post hoc Newman–Keuls test); semi-bold bar — median; box — interquartile range.