2022 / 01

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DOI: 10.24075/brsmu.2022.001

ORIGINAL RESEARCH

Meropenem-induced reduction in colistin susceptibility in Pseudomonas aeruginosa strain ATCC 27853

Savinova TA, Bocharova YuA, Chaplin AV, Korostin DO, Shamina OV, Mayanskiy NA, Chebotar IV
About authors

Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Tatiana A. Savinova
Ostrovityanova, 1, Moscow, 117997, Russia; moc.liamg@avonivasainat

About paper

Funding: the study was supported by the Russian Science Foundation (Project 20-15-00235).

Acknowledgements: the authors thank the Center of Precision Genome Editing and Genetic Technologies for Biomedicine of Pirogov Russian National Research Medical University for their advice on the methodology of the study.

Author contribution: Savinova TA — formal analysis of sequencing data, manuscript preparation; Bocharova YuA — methodology, formal analysis; Chaplin AV — formal analysis of sequencing data; Korostin DO — methodology, data validation; Shamina OV — methodology; Mayansky NA, Chebotar IV — concept; manuscript editing.

Received: 2021-12-27 Accepted: 2022-01-10 Published online: 2022-01-19
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Fig. A device (MEGA-plate, 40 × 20 cm) for spatiotemporal modeling of meropenem resistance in P. aeruginosa. The bottom of the device is divided into 5 sections separated by 2.5-cm walls. The culture medium in the MEGA plate has a sandwich composition. The bottom layer contains solid LB agar (the description is provided in the article) distributed into the sections. Each of the sections contains different concentrations of meropenem (shown on the right). The middle layer is LB agar without meropenem. The top layer is semi-solid LB agar (Miller). The arrow indicates the inoculation site and the direction in which P. aeruginosa propagates along the surface of semi-solid agar.
Table. Characteristics of carbapenem-resistant strains of P. aeruginosa with reduced susceptibility to colistin
Note: S — susceptible; I — intermediate; R — resistant; wt — wild type, matches the reference genome; SNP — single nucleotide polymorphism; * — according to the CLSI criteria, the term “susceptible” cannot be applied to describe the susceptibility of P. aeruginosa to colistin; all P. aeruginosa strains for which colistin MIC ≤ 2 µg/ml are classified as susceptible at increased exposure.