Methodology of determining the metabolomic profile of tumor-associated macrophages and monocytes in oncological diseases

About authors

1 Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

2 Laboratory of translational cellular and molecular biomedicine, National Research Tomsk State University, Tomsk, Russia

3 Laboratory of Genetic Technologies, Siberian State Medical University, Tomsk, Russia

4 Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

5 Institute of Transfusion Medicine and Immunology, MI3, Mannheim Faculty of Medicine, University of Heidelberg, Germany

6 German Red Cross Blood Service Baden-Württemberg–Hessen, Mannheim, Germany

Correspondence should be addressed: Vladimir E. Frankevich
Akademika Oparina, 4, Moscow, 117997, Russia; moc.liamg@hciveknarfv

About paper

Funding: the study was financially supported by the Russian Federation represented by the Ministry of Science and Higher Education of the Russian Federation (agreement dated 29 September 2021 № 075-15-2021-1073 on the topic "Genetic and epigenetic editing of tumor cells and the microenvironment in order to block metastasis ").

Author contribution: Frankevich VE, Kzhyshkowska JG — study planning and coordination, manuscript writing; Bragina OD — cancer patient selection and clinical characteristic preparation; Novoselova AV — sample preparation, HPLC-MS/MS; Larionova IV, Patysheva MR — monocyte and macrophage sample preparation and characterization, model TAM system set-up; Rakina MA — obtaining conditioned media of breast cancer cell lines; Starodubtseva NL — HPLC-MS/MS data analysis, manuscript writing; Frankevich VE, Larionova IV, Patysheva MR — discussion of results; Frankevich VE, Kzhyshkowska JG, Starodubtseva NL — manuscript editing.

Compliance with ethical standards: the study was approved by ethical review board of the Federal State Budgetary Educational Institution of Higher Education of the Siberian State Medical University of the Ministry of Health of Russia (protocol № 7 of 14 January 2017), federal laws of the Russian Federation (№ 152, 323, and others), and the 1964 Declaration of Helsinki.

Received: 2022-08-22 Accepted: 2022-09-13 Published online: 2022-10-13
Fig. 1. Characteristic total ion current chromatograms of lipid extracts from monocyte cells recorded in the positive ion mode. (A) Reversed-phase chromatography; (B) HILIC chromatography. PC — phosphatidylcholines; PE — phosphatidylethanolamines; SM — sphingomyelins; LPC — lysophosphatidylcholines; PE —hosphatidylethanolamines
Fig. 2. Time-averaged HPLC-MS mass spectrum of positive ions of a studied sample of macrophage lipid extract, obtained by reversed-phase chromatography
Table 1. Number of identified lipids in analyzed samples
Table 2. Areas of chromatographic peaks of amino acids obtained from the analysis of samples of monocytes and TAM