Ambient MS profiling of meningiomas: intraoperative oncometabolite-based monitoring

Bormotov DS1, Shamraeva MA1, Kuzin AA1, Shamarina EV1, Eliferov VA1, Silkin SV1, Zhdanova EV1, Pekov SI2,3, Popov IA1
About authors

1 Moscow Institute of Physics and Technology, Dolgoprudny, Russia

2 Skoltech, Moscow, Russia

3 Siberian State Medical University, Tomsk, Russia

Correspondence should be addressed: Stanislav I. Pekov
Bolshoy Blvd., 30, стр. 1, 121205, Moscow, Russia; ur.ewrof@vokep.valsinats

About paper

Funding: The study was conducted as part of the state task of the Ministry of Science and Higher Education (agreement №075-00337-20-02. project № 0714- 2020-0006), using the equipment of the N. N. Semenov Federal Research Center for Chemical Physics of the Russian Academy of Sciences.

Author contribution: Bormotov DS — experiment, data analysis, manuscript editing; Shamraeva MA — data analysis, preparation of illustrations, manuscript writing; Kuzin AA — experiment; Shamarina EV — experiment, ion source development; Eliferov VA — ion source development; Silkin SV — experiments, data analysis; Zhdanova EV — data analysis; Pekov SI — study concept, manuscript writing; Popov IA — search for sources of funding, project management, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of N. N. Burdenko National Medical Research Center of Neurosurgery (protocols №40 of 12 April 2016 and №131 of 17 July 2018) and conducted in accordance with the Declaration of Helsinki (2000 and subsequent revisions). All patients signed a voluntary informed consent form to participate in the study with use of biomaterials for research purposes.

Received: 2022-11-27 Accepted: 2023-01-14 Published online: 2022-12-30

The primary method of initial treatment of meningiomas is radical neurosurgical intervention. Various methods of intraoperative diagnostics currently in development aim to improve resection efficiency; we focus on methods based on molecular profiling using ambient ionization mass spectrometry. Such methods have been proven effective on various tumors, but the specifics of the molecular structure and the mechanical properties of meningiomas raise the question of applicability of protocols developed for other conditions for this particular task. The study aimed to compare the potential clinical use of three methods of ambient ionization in meningioma sample analysis: spray from tissue, inline cartridge extraction, and touch spherical sampler probe spray. To this end, lipid and metabolic profiles of meningioma tissues removed in the course of planned neurosurgical intervention have been analyzed. It is shown that in clinical practice, the lipid components of the molecular profile are best analyzed using the inline cartridge extraction method, distinguished by its ease of implementation and highest informational value. Analysis of oncometabolites with low molecular mass is optimally performed with the touch spherical sampler probe spray method, which scores high in both sensitivity and mass-spectrometric complex productivity.

Keywords: mass spectrometry, lipids, ambient ionization, electrospray ionization, meningioma, oncometabolites