ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Genetically encoded light-inducible sensor for nucleolar visualization
About authors
Skolkovo institute of science and technology, Center for Molecular and Cellular Biology, Moscow, Russia
Correspondence should be addressed: Lidia V. Putlyaeva
Bolshoy Boulevard, 30, Moscow, Russia, 121205; ur.liam@avoliahkim.aidil
About paper
Funding: this work was supported by the Russian Science Foundation grant № 22-24-01109.
Received: 2023-11-27
Accepted: 2023-12-11
Published online: 2023-12-17
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Fig. 1. Characterization of the genetically engineered LANS system enabling light-dependent recruitment of LANS2 to nucleoli. A. Schematic representation of the lightdependent dimerization probes LANS1 and LANS2. FP=fluorescent protein. В. Fluorescence microscopy images of HeLa cells transfected with LANS1 and LANS2 before (left), during (middle), and after (right) blue light irradiation. White arrows indicate the nucleoli. С. Fluorescence image of the nucleolus in HeLa cell expressing the DPF3-mScarlet sensor. D. Graph showing the nucleolus/cytoplasm relative fluorescence ratio for LANS2 during the experiment. Data represent mean ± range (n = 33 nucleoli)
