ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Discriminatory power of multiplex PCR for detection of mycobacterial co-infection
About authors
1 Central Tuberculosis Research Institute, Moscow, Russia
2 Yevdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia
Correspondence should be addressed: Tatiana G. Smirnova
Yauza alley, 2, str. 1A, Moscow, 107564, Russia; ur.liam@aktat_s
About paper
Funding: the study was conducted within the framework of the State Assignment of the Central Tuberculosis Research Institute, R&D project No. 122041100246-3 "Interspecific and intraspecific polymorphism of mycobacteria in patients with tuberculosis and mycobacteriosis receiving specific therapy"
Author contribution: Smirnova TG — experimental procedure (real-time PCR), data analysis, manuscript draft; Andreevskaya SN — literature review, data interpretation, review of publications on the issue; Ustinova VV — PCR; Larionova EE — data analysis; Kiseleva EA — model sample preparation; Chernousova LN, Ergeshov A — developing the study design; all authors contributed to discussion
Received: 2024-06-21
Accepted: 2024-07-16
Published online: 2024-08-04
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Table 1. The layout of the PCR strip and fluorescence detection channels
Table 2. Characteristics of model DNA samples
Table 3. Results of testing of model samples of the DNA mixture of two mycobacterial species by multiplex PCR
