ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
In vivo assessment of the role of liver metabolism in sydnone imine biotransformation
About authors
1 Tver State Medical University, Tver, Russia
2 Research Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Nikita S. Popov
Sovetskaya, 4, Tver, 170100, Russia; ur.liam@vopop.sn
About paper
Funding: the study was carried out under the state assignment for the research project “Development of the Candidate Drug Possessing Mostly Central Vasodilatory Activity for Treatment of Cerebrovascular Disorders”, state registration number 124020900020-4.
Author contribution: Popov NS — search for the BBP2023 compound metabolites, bioanalytical method development, manuscript writing; Terekhov VM — developing the design of vascular liver isolation in rats, surgical procedure; Baranov MS — synthesis of the BBP2023 compound and its metabolites, manuscript writing; Balabanyan VYu — goal setting, developing the study design, manuscript writing, Kaurova DE — literature review, manuscript writing; Myasnyanko IN — synthesis of the BBP2023 compound and its metabolites, manuscript writing; Terekhova EA — developing the design of vascular liver isolation in rats, surgical procedure; all authors contributed to the publication equally, they confirm compliance of authorship with the ICMJE international criteria.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Tver State Medical University (protocol No. 5 dated 19 June 2024). All experiments were conducted in accordance with the principles of Good Laboratory Practice (Order of the Ministry of Health of the Russian Federation No. 708n of 23.08.2010, Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes).
Received: 2025-04-16
Accepted: 2025-04-30
Published online: 2025-05-15
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Fig. 1. BBP2023 structural formula, where R is a saturated branched hydrocarbon radical (С7H15)
Fig. 2. A. Skeletonization of the abdominal aorta (aorta abdominalis) with the celiac trunk (truncus celiacus) B. Stumps of the celiac trunk with the ligatures placed. C. Portal vein (vena portae) isolation. D. Portal vein with the ligatures placed. E. Crossing the portal vein between the ligatures. F. Drug injection into the inferior vena cava (vena cava inferior). A sterile latex glove was used as a contrast medium during surgery in order to enhance the information content
Fig. 3. Fragments of blood plasma chromatograms of the sham-operated animals, which were intravenously administered the BBP2023 compound in a dose of 1/100 LD50. R — saturated branched hydrocarbon radical (С7H15)
Fig. 4. Fragments of blood plasma chromatograms of the rats with vascular liver isolation, which were intravenously administered the BBP2023 compound in a dose of 1/100 LD50. R — saturated branched hydrocarbon radical (С7H15)
Table 1. Expected BBP2023 compound biotransformation products and their monoisotopic mass
Note: R — saturated branched hydrocarbon radical (С7H15)
Table 2. Chromatographic parameters for determination of the expected BBP2023 compound biotransformation products
Table 3. Structural formulae of the identified BBP2023 compound metabolites and MRM transitions used for detection during chromatographic analysis
Note: R — saturated branched hydrocarbon radical (С7H15).
