2025 / 06

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DOI: 10.24075/brsmu.2025.063
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Anti-opisthorchiasis and hepatoprotective effects of Populus tremula bark extract: in vitro studies

Perina EA1 , Buyko EE1 , Kaidash OA1 , Efimova LF1 , Diksas EB1 , Krivoshchekov SV1 , Ivanov AA1,2 , Ivanov VV1 , Udut EV1
About authors

1 Central Research Laboratory, Siberian State Medical University оf the Ministry of Health of the Russian Federation, Tomsk

2 Biolit limited liability company, Tomsk

Correspondence should be addressed: Ekaterina Alexandrovna Perina
Moskovsky tract, 2, bldg. 16, Tomsk, 634050, Russia; moc.liamg@anirepenirehtac

About paper

Funding: the project is supported by the state research assignment "720000F.99.1.BN62AB05000 Development of a new drug based on a natural complex of phenol glycosides and arabinogalactanes for the treatment of trematodosis" (Agreement on granting subsidies to a federal budget-supported or autonomous institution as financial support for the state assignment involving provision of public services (implementation of works) of 21.01.2025 No. 056-03-2025-100, supplementary agreement of 04.04.2025 No. 056-03-2025-100/1).

Author contribution: Perina EA — reproduction of opisthorchiasis invasion, assessment of anti- opisthorchiasis effect; Buyko EE — determination of hepatoprotective effect and cytotoxicity of the extract; Kaidash OA — statistical data processing, article authoring, final review and approval of the manuscript; Efimova LV — assessment of damage to the maritae's tegument; Diksas EB — reproduction of opisthorchiasis invasion; Krivoshchekov SV — synthesis of the extract; Ivanov AA — chemical analysis of the extract; Ivanov VV — study design and conceptualization, data analysis and interpretation; Udut EV — study coordination, final review and approval of the manuscript.

Compliance with ethical standards: the study was approved by the Institutional Animal Care and Use Committee of the Preclinical Research Center, Central Research Laboratory, Siberian State Medical University of the Ministry of Health of the Russian Federation (Resolution No. 1 of September 02, 2023). The work was performed in full compliance with current drug circulation regulations, guidelines for the use of laboratory (experimental) animals in preclinical (non-clinical) studies, and sanitary and epidemiological standards.

Received: 2025-10-17 Accepted: 2025-11-19 Published online: 2025-12-02
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