ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2026 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Immunomodulatory effects of the placental conditioned medium and HLA-DR-dependent mechanisms in carriers of the HLA-DRB1*01:01 alleles with preeclampsia
About authors
1 V.I. Vernadsky Crimean Federal University, Simferopol, Russia
2 Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia
Correspondence should be addressed: Leya E. Sorokina
Akademika Oparina, 4, Moscow, 117198, Russia; ur.liam@anikoros.ayel
About paper
Author contribution: Sorokina LE — data acquisition, analysis, and interpretation, manuscript writing; Krasnyi AM — data acquisition, analysis, and interpretation, statistical data processing; Fomochkina II — study concept and design, manuscript writing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (protocol No. 11 dated November 11, 2021).
Received: 2026-05-10
Accepted: 2026-06-20
Published online: 2026-06-28
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Fig. 1. Research design. CD14⁺ monocytes and CD4⁺ Т cells were isolated from peripheral blood of pregnant women. The placental conditioned medium (PCM) was obtained by culturing placental explants for 24 h. Cells were co-cultured under various experimental conditions with subsequent assessment of the CD4⁺CD25⁺FoxP3⁺ Treg cell counts by flow cytometry and the cytokine concentrations in supernatants by ELISA
Fig. 2. Percentage of CD4⁺CD25⁺Foxp3⁺ Treg cells in the CD4⁺ T cell population under various co-culture conditions in the index group and comparison group. Data are presented as Me (Q1–Q3). The nonparametric Mann–Whitney U-test was used to compare independent groups; the Wilcoxon test for related samples was used for intragroup comparisons
Fig. 3. Cytokine profile of supernatant under various co-culture conditions. IL17 (A), TNFα (B), IFN-γ (C), IL10 (D), and IL4 (E) levels in the index group and comparison group are provided. Data are presented as Me (Q1–Q3). The nonparametric Mann–Whitney U-test was used to compare independent groups; the Wilcoxon test for related samples was used for intragroup comparisons
