Original research Analysis of mildronate effect on the catalytic activity of cytochrome Р450 3А4
Kuzikov AV, Bulko TV, Masamrekh RA, Makhova AA, Archakov AI, Usanov SA, Shikh EV, Shumyantseva VV
In this work, we have studied the effect of mildronate on the catalytic properties of cytochrome Р450 3А4. The analysis of the catalytic activity was carried out using electrochemical methods, with cytochrome Р450 3А4 immobilized on the electrode surface. In the presence of 50 μM mildronate, no increase was observed in the turnover number of cytochrome Р450 3А4-dependent N-demethylation of erythromycin. The values of the turnover number kcat calculated from the product formed by the reaction were 6.1 ± 0.6 min–1 (Р450 3А4 + erythromycin) and 5.5 ± 1.4 min–1 (Р450 3А4 + erythromycin + mildronate). Thus, electroanalysis of cytochrome Р450 3А4 catalytic activity demonstrated the possibility of a safe and effective complex drug therapy with concurrent administration of mildronate and the macrolide (erythromycin).
Received: 2016-11-28
Published online: 2017-01-19
DOI: 10.24075/brsmu.2016-06-02
Comments 0
VIEWS 4043
Method Biocompatible doxorubicin-loaded superparamagnetic iron oxide nanoparticles: synthesis and antineoplastic activity
Starostin KM, Olenin AYu, Fedotcheva TA, Shirokikh KE, Lisichkin GV, Shimanovskii NL
Abstract has not been added yet...
Received: 2015-09-16
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3158
Method High sensitivity and high specificity inflammation detector
Titov VY, Kreynina MV, Petrov VA, Osipov AN, Kamchatnov PR, Shalina RI
Abstract has not been added yet...
Received: 2015-09-13
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3268
Original research Combining phases II and III in а clinical trial of “Hexasens”: assessment and reasoning
Ivanova-Radkevich VI, Machinskaya EA, Filonenko EV, Lukianets EA
Abstract has not been added yet...
Received: 2015-10-02
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3192
Original research Reference ranges for standard coagulation tests and thrombodynamics assay during normal pregnancy at various gestational ages
Voroshilina ES, Ovsepyan RA, Plotko EE, Gerasimova OB, Baskova OYu, Budykina TS, Vuimo TA
Abstract has not been added yet...
Received: 2015-10-08
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3244
Original research Association between the activity of CYP2D6 enzyme and profile of efficacy and safety of haloperidol in patients with alcohol addiction
Sychev DA, Zastrozhin MS, Smirnov VV, Savchenko LM, Bryun EA, Guschina YuSh, Sorokin AS, Aguzarov AD
Abstract has not been added yet...
Received: 2015-10-06
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3070
Original research High carrier frequency of CFTR gene mutations associated with cystic fibrosis, and PAH gene mutations associated with phenylketonuria in Russian population
Abramov DD, Kadochnikova VV, Yakimova EG, Belousova MV, Maerle AV, Sergeev IV, Ragimov AA, Donnikov AE, Trofimov DYu
Abstract has not been added yet...
Received: 2015-09-11
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3310
Original research Association of the IL10 gene polymorphism with the development of persistent oligoarticular juvenile idiopathic arthritis
Nazarova LSh, Danilko KV, Malievsky VA, Viktorova TV
The aim of the study was to determine whether the IL10 –592C>A (rs1800872) polymorphism is associated with the development of persistent oligoarticular juvenile idiopathic arthritis (JIA) in children from Bashkortostan, Russia. Genotyping of 107 patients with persistent oligoarticular JIA and 206 healthy controls was performed by PCR-RFLP and real-time PCR methods. The IL10 –592CC genotype frequency was significantly higher in patients with persistent oligoarticular JIA than in controls (p = 0,033). After dividing the whole groups into subgroups based on gender there was found that the IL10 –592CC genotype and the IL10 –592C allele frequencies were significantly higher only in girls with persistent oligoarticular JIA in comparison to controls (p = 0,042 и p = 0,025, correspondingly). In this study we revealed the association of the IL10 –592C>A polymorphism with the development of persistent oligoarticular JIA in children from Bashkortostan, Russia.
Received: 2015-10-09
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3101