Bulletin of RSMU

ISSN Print 2500–1094 ISSN Online 2542–1204

Bulletin of RSMU

BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)

SCImago Journal & Country Rank

Log In Registration RU

Bogomilsky MR, Svitich OA, Gankovskiy VA, Rakhmanova IV

In this study we examined expression levels of TLR2, TLR4, TLR9 genes and НBD1, HBD2 and HNP1 antimicrobial peptides genes, which are part of the innate immunity, in children with grade II and grade III adenoid hypertrophy and healthy children. The patients were divided into three groups: those with indications for conservative treatment (group I, 39 participants), those with prior adenoidectomy (group II, 38 participants) and healthy controls (33 participants). Clinical material was presented by epithelial cells scraped from nasal mucosa (group I and control group) and pharyngeal tonsils scrapes (group II). No significant difference in gene expression based on the type of biological material was observed. The expression levels of the genes inspected in group I did not differ significantly from those in the control group. Group II displayed a significantly increased expression of TLR2 and TLR4 genes (15.4 and 10.3 times respectively with p <0.05) in comparison with the control group, with TLR9 gene expression level being 5 times lower, HBD1 gene expression — 33.4 times lower, HBD2 gene expression — 21 times lower, HNP1 gene expression — 3.4 times lower. Such defects in defense mechanisms can lead to disease complications and be an additional indication for surgery.

Received: 2015-09-14

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3310

Gankovskaya LV, Svitich OA, Khamaganova IV, Gyulaliev DM

The role of innate immune mechanisms in the pathogenesis of localized scleroderma is still not understood. However molecules of innate immunity such as toll-like receptors and cytokines are increasingly seen as a driver for sclerosis development in patients with localized scleroderma. The objective of this study was to investigate the expression of TLR2, HBD-1 and TNF-α genes both in the lesions and unaffected skin areas in patients with localized scleroderma. This study was real time PCR based. It enrolled 63 patients with localized scleroderma including 49 individuals with plaque morphea, 8 individuals with idiopathic atrophoderma of Pasini and Pierini, and 6 individuals with co-occurring plaque morphea and lichen sclerosus. The control group consisted of 8 healthy donors. The study showed the imbalance of innate immune factors in the lesion areas with the reduction in TLR2 gene expression and increase in HBD-1 and TNF-α genes expression compared to healthy donors skin. These changes in the innate immune factors can indicate defects in the processes of pathogene and endogenous ligand recognition, local inflammation development as a result of increased TNF-α expression, and fibroblast activity mediated by a high level of HBD-1 antimicrobial peptide.

Received: 2015-09-11

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3301

Galimova ES, Khusnutdinova EK

The objective of this study is to analyze the associations of rs1554286 (+1547 C/T) polymorphism in IL10 gene and the risk of psoriasis development. DNA samples were collected from 273 patients with psoriasis and 298 healthy controls. Genotyping of rs1554286 polymorphic locus in IL10 gene was performed by real-time polymerase chain reaction using the CFX 96™ Real-Time Cycler (BioRad, USA). In the total sample of patients with psoriasis the association of rs1554286 T allele (OR = 0.72; 95 % CI 0.54–0.96) of IL10 gene and a lower risk of disease development was found. It was established that rs1554286 Т allele of IL10 gene (OR = 0.54; 95% CI 0.30–0.97) marks the lower risk of the disease development in patients with severe psoriasis. Besides the carriage of T allele and the heterozygous C/T rs1554286 genotype of IL10 gene reduces the risk of disease development in patients with type I psoriasis (OR = 0.69; 95 % CI 0.51–0.95; OR = 0.63; 95 % CI 0.43–0.93 respectively). The study identified the rs1554286 polymorphic locus in IL10 gene as a marker of a lower risk of psoriasis development in the Russian population of Volgo-Ural region.

Received: 2015-09-13

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3488

Lyadova MV, Tuchik ES, Lyadova AV

The article is devoted to the problem of trauma doctors' legal awareness of emergency medical assistance. Based on the results of sociological investigations, there were demonstrated the main causes of conflicts between doctors and patients and the ways to prevent them.

Received: 2015-02-17

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 2908

Komissarova OG, Konyaeva OO, Berezhnaya OO, Abdullaev RYu, Vasilyeva IA

The aim of the study was the comparison of the characteristics of clinical manifestations and efficacy of treatment of patients with pulmonary tuberculosis with multiple and extensively drug-resistant M. tuberculosis combined with different types of diabetes mellitus. There were studied 41 patients with pulmonary tuberculosis combined with diabetes mellitus type 1 and 30 patients with pulmonary tuberculosis combined with diabetes mellitus type 2. It was established that patients with diabetes mellitus type 1 often diagnosed infiltrative pulmonary tuberculosis, while in patients with diabetes mellitus type 2 it was often found fibrous-cavernous pulmonary tuberculosis. Efficiency of complex treatment for bacterial excretion extinction in the two groups was not significantly different (73.2 and 66.7 %, respectively). However, the treatment for closing of cavities in the lung was more effective in patients with diabetes mellitus type 1 (accordingly 61.0 and 36.6 %), which was probably due to the peculiarities of tuberculosis process in those patients.

Received: 2015-02-03

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3125

Radtsig EYu, Zlobina NV, Lapitskaya AS, Selkova EP

The article presents data on the etiology of acute adenoids against ARVI in children based on the results of microbiological (bacteriological, virological, mycological) study. Frequency of occurrence of various viral and bacterial pathogens is presented.

Received: 2015-04-07

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 2830

Kotova EN, Bogomilsky MR

The article presents the literature and own data on the diagnosis and methods of surgical intervention for congenital choanal atresia in children. It is described, the method of endoscopic transnasal choanotomy, applied by the authors. A new variant of the stent used for the formation of choanal stoma in the postoperative period with the aim of increasing the effectiveness of surgical intervention and prevention of restenozation is suggested.

Received: 2015-04-07

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3153

Gamidov SI, Polivoda MD, Krasova OM, Popova AYu, Dubova EA, Pavlov KA

The aim of the study was to estimate the effect of the level and duration of the obstruction of the seminal tract on the morphological and functional state of the testicular tissue. 50 adult outbreed male laboratory rats were observed in the study. The rats were divided into 4 groups: 1 — vas deferens obstruction proximally, 2 — vas deferens obstruction distally, 3 — epididymis obstruction, 4 — controls (intact animals). The testes and epididymis were removed in 3 and 6 months for histological examination. Testicular volume and spermatogenesis status decreased in all groups. Also, these changes depend on the level and duration of the obstruction of the seminal tract.

Received: 2015-02-04

Published online: 2017-01-05

DOI:

Comments 0

VIEWS 3003