2014 / 03

Copyright: © 2017 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Psoralen Photosensitized Damage of Erythrocyte Membranes, Effects of Bivalent Cations and Fluence Rate of UVA-Irradiation: a Literature Review

Lysenko EP, Potapenko AYa
About authors

Department of Physics and Mathematics, Pediatric Faculty,
Pirogov Russian National Research University, Moscow, Russia

Correspondence should be addressed: Evgeniy Lysenko
ul. Ostrovityanova, d. 1, Moscow, Russia, 117997; ur.liam@1oknesyle

Received: 2014-06-06 Accepted: 2014-09-17 Published online: 2017-01-05
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Psoralens combined with UVA-irradiation (320–400 nm) are used for the treatment of skin and autoimmune diseases (PUVA-therapy). One possible mechanism of therapeutic and side phototoxic effects of PUVA-therapy can be realized through the stage of formation of psoralen photooxidation products (POP-products). In the present paper, the mechanisms of formation of two types of POP-products are discussed. POP1-products are predominantly formed in solutions at low psoralen concentration and/or under low fluence rate of UVA-irradiation. These POPi-products possess immunosuppressive activity and are detected by spin trap EPR method. POP2-products are formed under high fluence rate of UVA-irradiation and/or at high psoralen concentration. They possess hemolytic activity and are detected by Fe(ll)-induced chemiluminescence. The influence of Fe(ll)-ions on POP-induced membranotoxic effects is discussed.

Keywords: chemiluminescence, psoralen, fluence rate of UVA-irradiation, photooxidation, hemolysis, Fe(ll)-ions, spin traps

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