A study of the role of Prevotella intermedia in the development of chronic periodontitis using real-time polymerase chain reaction

About authors

1 Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia

2 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

3 Sechenov First Moscow State Medical University, Moscow, Russia

4 Kursk State University, Kursk

5 Central Research Institute of Dental and Maxillofacial Surgery, Moscow

6 Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow

Correspondence should be addressed: Alexei B. Shevelev
27 km Kievskoe highway, Institute of Poliomielit village, Moscow, 142782; moc.liamtoh@a_levehs

About paper

Funding : the work was done with the financial support of the Ministry of Education and Science of the Russian Federation (state task No. 19.1724.2014 / K in the field of scientific activity).

Received: 2015-09-13 Accepted: 2015-10-02 Published online: 2017-01-05

In a real-time polymerase chain reaction-based assay with a Dentoflor kit Prevotella intermedia, a periodontal pathogen, was found to be present in 55 % of patients with chronic periodontitis. This percantage is significantly lower than the corresponding statistic for Porphyromonas gingivalis, Tannerella forsuthensis and Treponema denticola. In addition, hypercolonization of periodontium by P. intermedia was detected in 7.7 % of patients in the control group. This suggests that P. intermedia hypercolonization is an important prognostic factor indicating the severity of periodontal disease. The abnormal increase in P. intermedia is rarely a result of a monoinfection. Compared to other “red complex” pathogens, this species is more likely to co-occur with P. gingivalis, T. forsythensis and T. denticola, which leads to the most severe forms of chronic periodontitis. Unlike other “red complex” pathogens, P. intermedia showed no significant difference in occurrence and the degree of periodontal damage in male and female individuals with equal colonization levels.

Keywords: microbiome, polymerase chain reaction, chronic periodontitis, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola