Fig. 1. Location of fatty acid- and Cu-binding sites; location of probes I and II in domains I, II and III in HSA structure

Fig. 2. Fluorescence spectra obtained in the experiments. (1) HSA (0.66 μm) and AAPH (2.5 mM), figures next to curves show incubation time. (2) HSA (0.6 μm), Н₂О₂ (3 mM) and Со²⁺ (0.3 mM), figures next to HSA + Н₂O₂ + Со²⁺ curves show time after introducing Со²⁺ to the system: 0, 3, 6, 9 and 15 minutes. (3) HSA (0.6 μm), figures next to curves show UV-irradiation dosage, kJ/cm². (4) HSA (fraction isolated from blood plasma and diluted 1:100) combined with neutrophils stimulated by barium sulfate and FMLP

Fig. 3. Changes in fluorescent and antioxidant properties of HSA exposed to different doses of UV-irradiation. (1) HSA fluorescence spectra (0.66 μm); the protein was exposed to different doses of UV-irradiation (figures show the dosage, J/cm²: 0 — native protein, 1 — 0.050, 2 — 0.200, 3 — 0.400, 4 — 0.600, 5 — 0.800, 6 — 1.000). (2) Chemiluminescence curves of HSA (0.66 μm) exposed to different doses of UV-irradiation (figures next to curves show the dosage, J/cm²) in the system containing phosphate buffer solution (PB) (37 °С), AAPH (2.5 mM), luminol (Lum) (2 μm), system total volume 1.000 ml

Fig. 4. Comparison of HSA fluorescence changes (0.66 μm) (λ³⁴⁰ _max = 337 nm) and the increase in the total antioxidant activity (t_lat, min) with the increased dosage of short wavelength radiation, J/cm²