2016 / 03

Next Paper
DOI: 10.24075/brsmu.2016-03-02

METHOD

Modification of the method for analysis of genome editing results using CRISPR/Cas9 system on preimplantation mouse embryos

Dimitrieva TV1, Reshetov DA1, Zhernovkov VE1, Vlodavets DV3, Zotova ED1, Ermolkevich TG1,2, Deykin AV1,2
About authors

1 Marlin Biotech, Moscow, Russia

2 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

3 Department of Neurology, Neurosurgery and Medical Genetics, Medical Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Tatiana Dimitrieva
ul. Vavilova, d. 34/5, Moscow, Russia, 119334; moc.liamg@at.aveirtimid

About paper

Acknowledgements: authors thank the Shared Resource Center of the Institute of Gene Biology of Russian Academy of Sciences for the equipment provided for this research.

Received: 2016-06-16 Accepted: 2016-06-21 Published online: 2017-01-05
|
  1. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 2013; 153 (4): 910–8.
  2. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337 (6096): 816–21.
  3. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013; 339 (6121): 819–23.
  4. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, et al. RNA-guided human genome engineering via Cas9. Science. 2013; 339 (6121): 823–6.
  5. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell. 2013; 154 (6): 1370–9.
  6. Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M. Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013; 3: 3355.
  7. Li D, Qiu Z, Shao Y, Chen Y, Guan Y, Liu M, et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol. 2013; 31 (8): 681–3.
  8. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, et el. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell. 2014; 156 (4): 836–43.
  9. Mou H, Kennedy Z, Anderson DG, Yin H, Xue W. Precision cancer mouse models through genome editing with CRISPR-Cas9. Genome Med. 2015; 7 (1): 53.
  10. Carroll KJ, Makarewich CA, McAnally J, Anderson DM, Zentilin L, Liu N, et al. A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9. Proc Natl Acad Sci U S A. 2016; 113 (2): 338–43.
  11. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, et al. Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014; 32 (9): 941–6. DOI: 10.1038/nbt.2951.
  12. Fujii W, Kawasaki K, Sugiura K, Naito K. Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013; 41 (20): e187. DOI:  10.1093/nar/gkt772. Epub 2013 Aug 30.
  13. Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T. A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice. BMC Biotechnol. 2014; 14: 69. DOI: 10.1186/1472-6750-14-69.
  14. Hou Y, Wu K, Shi X, Li F, Song L, Wu H, Dean M, et al Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing. Gigascience. 2015; 4: 37.
  15. Montague TG, Cruz JM, Gagnon JA, Church GM, Valen E. CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res. 2014; 42 (Web server issue): W401–7.
  16. Green MR, Sambrook J. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells. In: Green MR, Sambrook J, editors. Molecular Cloning: A Laboratory Manual. 4th ed. New York: Cold Spring Harbor Laboratory Press; 2012.
  17. Peciña A, Lozano Arana MD, García-Lozano JC, Borrego S, Antiñolo G. One-step multiplex polymerase chain reaction for preimplantation genetic diagnosis of Huntington disease. Fertil Steril. 2010; 93 (7): 2411–2.
  18. Tsuchiya S, Sueoka K, Matsuda N, Tanigaki R, Asada H, Hashiba T, et al. The “Spanning Protocol”: A new DNA extraction method for efficient single-cell genetic diagnosis. J Assist Reprod Genet. 2005; 22 (11–12): 407 –14.
  19. Sakurai T, Kamiyoshi A, Watanabe S, Sato M, Shindo T. Rapid zygosity determination in mice by SYBR green real-time genomic PCR of a crude DNA solution. Transgenic Res. 2008; 17 (1): 149–55.