ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
Library preparation for metagenomic sequencing with Illumina
About authors
1 Genotek Inc., Moscow
2 Moscow South-West High School No. 1543, Moscow, Russia
Correspondence should be addressed: Anna Krasnenko
Nastavnicheskiy per. 17, str. 1, pod. 14, Moscow, 105120; moc.liamg@oknensarkanna
About paper
Acknowledgements: the authors thank Daria Plakhina and Ivan Stetsenok of Genotek for their help and Sergey Glagolev of Moscow South-West High School No. 1543 for his valuable advice and comments.
Contribution of the authors to this work: Krasnenko AYu, Eliseev AYu — analysis of literature, research planning and implementation, data analysis and interpretation; Borisevich DI, Tsukanov KYu — bioinformatic analysis; Davydova AI — drafting of a manuscript; Ilinsky VV — research planning, scientific advisor. All authors participated in editing of the manuscript.
Received: 2017-04-12
Accepted: 2017-04-24
Published online: 2017-05-31
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Fig. 1. Agarose gel with PCR products after electrophoresis. The first well contains a DNA-size marker, the rest contain the samples. Primer dimers (PCR by-products) are marked with a black oval
Table 1. Universal primer pairs consist of a sequence complementary to the V3 and V4 regions of the 16S rRNA gene and a synthetic sequence complementary to Nextera and Truseq adapters
Table 2. Parameters of PCR for amplification of the V3 and V4 regions using universal primers for the Nextera adapter
