ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
A study of the repertoire of activated T-cell clones obtained from a patient with ankylosing spondylitis
About authors
Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Ivan V. Zvyagin
ul. Ostrovityanova, d. 1, Moscow, Russia, 117997; moc.liamg@nigayvzi
About paper
Funding: this work was supported by the Ministry of Education and Science of the Russian Federation, Project ID RFMEFI60716X0158.
Acknowledgements: we are grateful to the patient who has kindly given his consent to participate in the study; to Denis Fedorenko, a hematologist and Professor of Maximov Hematology and Cell Therapy Department (Pirogov National Medical Surgical Center) for his consultations; Elena Kovalenko, a senior researcher at Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, for her assistance in conducting a flow cytometry analysis.
Received: 2017-12-15
Accepted: 2017-12-25
Published online: 2018-02-16
|
Fig. 1. Comparison of clonal composition of different T-cell subpopulations. Percent of the clonal diversity (shown in red) and percent of the T-cell repertoire (shown in black) represented by clonotypes shared by two compared samples. *F1, *CD4, *CD8 are repertoires of F1, CD4, and CD8 samples, respectively, reconstructed on the basis of 17, 523 randomly in silico selected TCR cDNA molecules (that equals to the number of TCR cDNA molecules covered by the analysis of the CD3+CD38+HLA-DR+ subset)
Fig. 2. Distribution of the clonotypes identified in the CD3+CD38+HLA-DR+ subset depending on their abundance in the total repertoire (F1). The Y axis represents the proportion of clonotypes in the studied subset relative to the total number of clonotypes in the subset; the X axis represents the abundance of clonotypes in the total repertoire of T-cells
Fig. 3. Abundance of the clonotypes associated with AS and found in the synovial fluid of patients with AS in the repertoire of activated T-cells. Rank (reference number) in the repertoire of activated T-cells for each clonotype from the list (see explanation in the text), is represented by green vertical line
Table 1. Comparison of CD3+CD38+HLA-DR+ clonal diversity with the total repertoire of peripheral blood T-cells
Note. * — association with the subset of cytotoxic of helper T-cells based on the analysis of CD8+ and CD4+ samples’ repertoires; ** — percent of the total clonal diversity of the studied subset; *** — the number of clonotypes detected only in the CD3+CD38+DR+ subset.
Table 2. Analysis of frequency in the peripheral blood and synovial fluid samples of patients with AS for the clonotypes identified in CD3+CD38+HLA-DR+ subset
Note. * — The list includes clonotypes of the CD3+CD38+HLA-DR+ cell subset isolated from the peripheral blood of the patient that were not found in the repertoires of healthy HLA-B*27 positive donors; ** — the number of peripheral blood samples of AS patients, where the clonotype was identified, is specified in brackets; *** — association with the subset of cytotoxic of helper T-cells based on the analysis of the repertoire of CD8+ and CD4+ samples.
