ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2018 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Physical and chemical properties of recombinant kpp10 phage lysins and their antimicrobial activity against Pseudomonas aeruginosa
About authors
1 Laboratory for Translational Biomedicine,
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow
2 Faculty of Fundamental Medicine,
Lomonosov Moscow State University, Moscow
3 Center for Strategic Planning of the Ministry of Health of the Russian Federation, Moscow
4 Department of Virology, Faculty of Biology,
Lomonosov Moscow State University, Moscow
Correspondence should be addressed: Vladimir A. Gushchin
Gamaleya 18, Moscow, 123098; moc.liamg@adainawow, gro.ayelamag@nihchsug.a.rimidalv
About paper
This work was supported by the Ministry of Education and Science of the Russian Federation as part of the project RFMEFI60117X0018.
Received: 2018-02-02
Accepted: 2018-03-23
Published online: 2018-04-27
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Fag. 1.Physical and chemical properties of endolysin L-KPP10 and artilysin AL-KPP10. Circular dichroism spectra of AL-KPP10 and L-KPP10 (A). Spectra of tryptophan fluorescence of proteins under denaturing and native conditions (B). Dynamic laser light scattering in the solutions of native AL-KPP10 (C) and L-KPP10 (D)
Fig. 2. Antimicrobial activity of the recombinant endolysin against strain РА103 of Pseudomonas aeruginosa. Treatment with different concentrations of the recombinant L-KPP10 in the absence of permeabilizing agents (A). 0.5 mM EDTA added to the bacteria combined with L-KPP10 (B). * — Difference is significant at р = 0.05
(Mann-Whitney U)
Fig. 3. Antimicrobial activity of the recombinant artilysin AL-KPP10 against sensitive strains of Pseudomonas aeruginosa. Treatment of Pseudomonas aeruginosa sensitive strains with different concentrations of recombinant AL-KPP10 in the absence of permeabilizing agents (А), р < 0.01 (Mann-Whitney U). Electron microscopy of Ts 43-16 (clinical isolate of Pseudomonas aeruginosa) following incubation with 50 μg/ml AL-KPP10 for 10 min, 20 min and 30 min (B). A Petri dish with artilysin and clinical isolate Ts 49-16 of Pseudomonas aeruginosa upon incubation for 30 min (C)
Table 1. Antimicrobial activity of artilysin AL-KPP10 against clinical isolates of Pseudomonas aeruginosa
