The use of antimicrobial photodynamic therapy mediated by MC540 in the infected wound model

Shmigol TA1, Sobianin KA2, Prusak-Glotov MV1, Shchelykalina SP3, Nevezhin EV3, Yermolaeva SA4, Negrebetsky VV1
About authors

1 Department of Medicinal Chemistry and Toxicology,
Pirogov Russian National Research Medical University, Moscow

2 Laboratory of Biological Research. Institute for Translational Medicine,
Pirogov Russian National Research Medical University, Moscow

3 Department of Medical Cybernetics and Informatics, Biomedical Faculty,
Pirogov Russian National Research Medical University, Moscow

4 Laboratory for the Ecology of Pathogens,
Gamaleya Federal Research Center for Epidemiology and Microbiology, Moscow

Correspondence should be addressed: Tatiana Shmigol
Ostrovityanova 1, Moscow, 117997; moc.liamg@hsithsitat

About paper

Funding: this study was supported by the Russian Foundation for Basic Research (Project ID 16-33-00970 mol_a).

Received: 2018-01-20 Accepted: 2018-03-23 Published online: 2018-04-28

Photodynamic therapy (PDT) is an alternative to conventional therapies of infections. It can kill drug-resistant bacteria without damaging host tissues. In the present work we use the multiresistant strain PA21 of Pseudomonas aeruginosa to model a wound infection in mice and study the effect of PTD mediated by aqueous solutions of the anionic photosensitizer merocyanine 540 (solubilized in water and in 0.25 M sodium chloride) on bacterial decontamination and wound healing. To assess a therapeutic effect of PDT, we monitored bacterial contamination of the wound, measured the wound size in two planes using a caliper and carried out a histopathological examination of infected tissue sections. Our study reveals that PDT mediated by MC540 in the sodium chloride solution can induce bacterial death, inhibit bacterial re-growth and accelerate wound healing.

Keywords: wound healing, antimicrobial photodynamic therapy, multiresistant clinical strain, Pseudomonas aeruginosa, skin infection, soft tissue infection, merocyanine 540, wound infection