ORIGINAL RESEARCH
Conserved sequences of genes coding for the multidrug resistance pump AcrAB-TolC of Escherichia coli suggest their involvement into permanent cell “cleaning”
1 Research Centre for Medical Genetics, Moscow
2 Belozersky Institute of Physico-Chemical Biology,Lomonosov Moscow State University, Moscow
Correspondence should be addressed: Marina Karakozova
Microrayon 1, d.15, kv. 43, Zaraisk, Zaraiski r-n, Moscow region, 140601; moc.liamg@200257kvm
Acknowledgement: the authors wish to thank students (Dzhafarova T, Mamedova D, Maushev F, Miranian K, Omarova D and Rodionova E) and the Department of Morphology and Pathology of Medical Institute REAVIZ for the provided data, their analysis and discussion.
- Escherich T. Die Darmbakterien des Neugeborenen und Säuglinge. Fortschr. Med. 1885; 3: 515–522.
- Daegelen P, Studier FW, Lenski RE, Cure S, Kim JF. Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3). J Mol Biol. 2009; 394 (4): 634–43.
- Bachmann BJ. Pedigrees of Some Mutant Strains of Escherichia coli K-12. Bacteriological Reviews, 1972; 36 (4): 525–557.
- Lieb M, Weigle JJ, Kellenberger E. A study of hybrids between two strains of Escherichia coli. Journal of Bacteriology. 1955; 69 (4): 468–471.
- Lieb M. Forward and Reverse Mutation in a Histidine-Requiring Strain of Escherichia Coli Genetics. 1951; 36 (5): 460–477.
- Archer CT, Kim JF, Jeong H, Park JH, Vickers CE, Lee SY, et al. The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli. BMC Genomics. 2011; 12: 9.
- Vidal O, Longin R, Prigent-Combaret C, Dorel C, Hooreman M, Lejeune P. Isolation of an Escherichia coli K-12 mutant strain able to form biofilms on inert surfaces: involvement of a new ompR allele that increases curli expression. J Bacteriol. 1998; 180 (9): 2442–2449.
- Sulavik MC, Houseweart C, Cramer C, Jiwani N, Murgolo N, Greene J, et al. Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes. Antimicrob Agents Chemother. 2001; 45 (4): 1126–1136.
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, et al. Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006; 2: 2006.0007.
- Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006; 2: 2006.0008.
- BLASTp (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp& PAGE_TYPE=BlastSearch&LINK_LOC=blasthome)
- Szklarczyk D, Morris JH, Cook H, Kuhn M, Wyder S, Simonovic M, et al. The STRING database in 2017: quality-controlled protein- protein association networks, made broadly accessible. Nucleic Acids Res. 2017; 45: D362–68.
- Multiple Sequence Alignment Viewer v. 1.7.7 (https://www.ncbi. nlm.nih.gov/tools/msaviewer/about/)
- Nikaido H. Multidrug Resistance in Bacteria. Annu Rev Biochem. 2009; 78: 119–146.
- Martinez JL, Baquero F Mutation frequencies and antibiotic resistance. Antimicrob Agents Chemother. 2000; 44 (7): 1771– 1777.
- Moxon ER, Rainey PB. Nowak MA. Lenski RE. Adaptive evolution of highly mutable loci in pathogenic bacteria. Curr Biol. 1994; 4: 24–33.
- Lee H, Popodi E, Tang H, Foster PL. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci USA, 2012; 109 (41): E2774–E2783.
- Drake JW. A constant rate of spontaneous mutation in DNA- based microbes. Proc Natl Acad Sci USA, 1991; 88: 7160–7164.
- Sims GE, Kim S.-H. Whole-genome phylogeny of Escherichia coli/Shigella group by feature frequency profiles (FFPs) Proc Natl Acad Sci U S A. 2011; 108 (20): 8329–8334.
- Pos KM. Drug transport mechanism of the AcrB efflux pump. Biochim Biophys Acta. 2009; 1794 (5): 782–793.
- Nazarov PA, Osterman IA, Tokarchuk AV, Karakozova MV, Korshunova GA, Lyamzaev KG et al. Mitochondria-targeted antioxidants as highly effective antibiotics. Sci Rep. 2017; 7 (1): 1394.
- Piddock LJ Multidrug-resistance efflux pumps — not just for resistance. Nat Rev Microbiol. 2006; 4 (8): 629