ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2018 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
The efficacy of CRISPR-Cas9-mediated induction of the CCR5delta32 mutation in the human embryo
About authors
1 Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow
2 Lomonosov Moscow State University, Moscow, Russia
3 Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Denis V. Rebrikov
Ostrovityanova 1, Moscow, 117997; moc.liamg@vokirberd
Received: 2018-09-26
Accepted: 2018-10-09
Published online: 2018-10-16
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Fig. 1. gRNAs layout inside the human CCR5 gene
Fig. 2. Examples of real-time PCR curves for different genotypes: A — two control embryos, B — two mosaic embryos with about 3% of WT CCR5, C — two CCR5delta32 homozygous embryos
Table 1. gRNAs layout inside the human CCR5 gene
Table 2. Real-time PCR Cp values for each embryo
Note: no PCR products
