ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Investigation of the level of DNA double-strand breaks and mechanisms of cell death under irradiation of lung cancer and melanoma cells with ultra-high dose rate photon radiation
About authors
1 Russian Scientific Center of Roentgenoradiology, Moscow
2 Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow
3 Research Institute of Technical Physics and Automation of Rosatom, Moscow
Correspondence should be addressed: Vladimir K. Bojenko
Profsoyuznaya 86, Moscow, 117997; ur.liam@oknejobv
About paper
Funding: this work was supported by the Russian Science Foundation (Project 15-10355).
Received: 2018-08-15
Accepted: 2018-11-28
Published online: 2018-12-09
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Research into the effects of radiation delivered at ultrahigh dose rates > 1 × 107 Gy/min to biological objects is a new promising area of radiobiology. The unique characteristics of the high-current nanosecond electron accelerator Mir-M enable its use in medical and biological research, specifically in the experiments aimed at investigating the effect of therapeutic doses at a dose rate up to 100 MGy/s. In this work we study the effects of ultrahigh dose rate photon radiation on human lung carcinoma (A549) and melanoma (MelMtp-x) cells lines and compare them with those of the therapeutic gamma unit Rokus-AM. We show that ultrahigh dose rates induce more significant damage in the studied cell lines at doses between 2 and 7 Gy, radioresistant melanoma being more sensitive to photon radiation delivered at ultrahigh dose rates.
Keywords: apoptosis, photon radiation, gamma radiation, X-rays, ultrahigh dose rate, DNA double strand breaks